食品科学 ›› 2013, Vol. 34 ›› Issue (10): 173-176.doi: 10.7506/spkx1002-6630-201310037

• 分析检测 • 上一篇    下一篇

鱿鱼墨黑色素及黑色素铁定量分析

高 翔,李国云,王静凤,薛长湖,王玉明,李兆杰   

  1. 中国海洋大学食品科学与工程学院,山东 青岛 266003
  • 收稿日期:2012-03-01 修回日期:2013-03-26 出版日期:2013-05-25 发布日期:2013-05-07
  • 通讯作者: 李兆杰 E-mail:lizhaojie@ouc.edu.cn
  • 基金资助:

    海洋公益性行业科研专项

Quantitative Analysis of Melanin and Melanin-Fe (Ⅲ) from Squid

GAO Xiang,LI Guo-yun,WANG Jing-feng,XUE Chang-hu,WANG Yu-ming,LI Zhao-jie   

  1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
  • Received:2012-03-01 Revised:2013-03-26 Online:2013-05-25 Published:2013-05-07
  • Supported by:

    National Marine Public Welfare Scientific Research Project of China

摘要:

采用高速离心法从北太平洋鱿鱼(Ommastrephes bartrami)墨囊中提取鱿鱼黑色素,并通过黑色素与FeCl3螯合制备了Fe(Ⅲ)含量为7%的黑色素铁。采用H2O2氧化降解及高效液相色谱法定量测定黑色素两种降解产物2,3-二羧酸吡咯(PDCA)和2,3,5-三羧酸吡咯(PTCA)对鱿鱼墨黑色素及黑色素铁进行定量研究。结果表明:以H2O2氧化降解黑色素产物PDCA和PTCA为基础的鱿鱼墨黑色素定量方法效果良好。利用该方法对黑色素铁进行定量仅检测出40%的黑色素,盐酸脱铁后检出量上升至75%。常规的鱿鱼墨黑色素的定量方法不适用于黑色素铁的定量分析。

关键词: 鱿鱼墨黑色素, 鱿鱼墨黑色素铁, 定量分析, 高效液相色谱

Abstract:

Melanin was extracted from the ink of North Pacific squid (Ommastrephes bartrami) by high-speed
centrifugation. Squid melanin-Fe (Ⅲ) containing 7% (m/m) bound Fe (Ⅲ) was prepared by exposing squid melanin to
aqueous solution of FeCl3. Melanin was degraded with H2O2 and two degradation products, pyrrole-2,3-dicarboxylic acid
(PDCA) and pyrrole-2,3,5-tricarboxylic acid (PTCA) were determined by HPLC. The quantitative method to determine
squid melanin based on the degradation products of melanin PTCA and PDCA exhibited desired results. Only 40% of the
melanin was detected in quantitative analysis of the squid melanin-Fe (Ⅲ) by this method and increased to 75%. After
hydrochloric acid treatment to expel Fe, the melanin detected rose to 75%.

Key words: squid melanin, squid melanin-Fe (Ⅲ), quantitative analysis, HPLC

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