Abstract:

According to the specific sequence of genetically modified soybean line A2704-12, four sets of primers for loopmediated
isothermal amplification (LAMP) were designed. One set of highly specific primers was screened through optimizing
the reaction temperature and determining the specificity. An LAMP method for the detection of genetically modified soybean line
A2704-12 was developed after optimizing the reaction conditions and system. The specificity, sensitivity and stability of the LAMP
method were evaluated. The results showed that the LAMP method could detect specifically and stably transgenic soybean A2704-12
strain with a low detection limit of 0.1%. The results of detection the A2704-12 strain in soybean and its products by the LAMP
method were same as those from the real-time fluorescent PCR method.

Key words: loop-mediated isothermal amplification (LAMP), genetically modified soybean line A2704-12, specific detection