Protective Effect of Zeaxanthin on Tunicamycin-Induced Cell Cycle Arrest, Autophagy and Apoptosis of SH-SY5Y Cells

doi:10.7506/spkx1002-6630-20210325-318

Abstract

Abstract: Objective: To investigate the effect of co-treatment with zeaxanthin (Zea) and tunicamycin (TM) on the viability, cell cycle, autophagy and apoptosis of SH-SY5Y cells. Methods: The cells were divided into four groups: blank control (without drug treatment), Zea (5 μmol/L), TM-induced injury (5 μg/mL) and Zea (5 μmol/L) + TM (5 μg/mL). The methylthiazol tetrazolium (MTT) method was used to evaluate the survival rate of SH-SY5Y cells to determine the drug co-treatment time for further experiments; flow cytometry was used to determine the change of cell cycle; Western blot was used to determine the expression of autophagy-related proteins such as Beclin1, Beclin1-C and microtubule-associated protein 1 light chain 3B (LC3B), and apoptosis-related protein B-cell lymphoma 2 (BCL2). Results: Zea treatment (5 μmol/L) reduced the inhibitory effect of TM on the survival rate of SH-SY5Y cells. Compared to the injury group, a significant difference in the survival rate of SH-SY5Y cells was observed for the combined treatment group at 36 (P < 0.01) and 48 h (P < 0.05). In addition, compared to the blank control group, TM treatment displayed a significant difference in the survival rate of SH-SY5Y cells at 24, 36, and 48 h (P < 0.01). Compared to the blank group, TM treatment significantly increased the number of G0/G1 phase cells in the cell cycle (P < 0.01) and the expression of LC3B (P < 0.01), Beclin1 (P < 0.05) and Beclin1-C (P < 0.05), and significantly reduced the number of G2/M phase cells (P < 0.01) and the expression level of anti-apoptotic protein BCL2 (P < 0.01). The combined treatment alleviated the changes caused by TM, and significantly increased BCL2 protein expression compared to the injury group (P < 0.05). Conclusion: Zea can significantly extenuate TM-induced cell cycle G1 arrest, autophagy and apoptosis of SH-SY5Y cells, and its underlying mechanism is related to the modulatory of Zea on cell survival, cell cycle distribution, and the expression of autophagy and apoptosis-related proteins.

Key words: zeaxanthin, SH-SY5Y cells, cell cycle, autophagy, apoptosis

CLC Number:

TS201.4

ZHANG Lina, LI Mengjie, SHANG Yinghui, LIU Yunru, HUANG Hanchang, LAO Fengxue. Protective Effect of Zeaxanthin on Tunicamycin-Induced Cell Cycle Arrest, Autophagy and Apoptosis of SH-SY5Y Cells[J]. FOOD SCIENCE, 2022, 43(7): 112-119.

[1]	WANG Bin, CHEN Yilun, XIA Wenshui. Effect of Chitooligosaccharide on Ethanol-Induced Oxidative Stress Injury and Apoptotic Factors in Neonatal Rat Brain [J]. FOOD SCIENCE, 2022, 43(7): 105-111.
[2]	LIU Xiaoming, ZHANG Yuanyuan, LIN Xiaorong, GAO Xiong, ZHOU Weijian, LI Bin, CHEN Zhongzheng. Synergistic Anticancer Effect and Mechanism of Geraniol Combined with Oridonin on MCF-7 Cells [J]. FOOD SCIENCE, 2022, 43(3): 129-136.
[3]	SUN Pengzhen, CAO Jianxin, ZHAO Tianrui, CHENG Guiguang. Effect of Ultra-high Pressure Pretreatment on Antioxidant Capacity and Cytoprotective Activities against Oxidative Damage on HepG2 Cells of Peel of Passiflora edulis Sims [J]. FOOD SCIENCE, 2022, 43(1): 83-91.
[4]	LIU Mengdie, JIANG Huiwen, LI Jie, YAN Shoulei, WANG Qingzhang. Apoptotic Process of Fusarium oxysporum Spores Induced by Air Discharge [J]. FOOD SCIENCE, 2021, 42(9): 22-30.
[5]	XUE Hongkun, TAN Jiaqi, LI Qian, TANG Jintian. Procyanidin B2-Induced Apoptosis in MCF-7 Cells and Its Mechanism [J]. FOOD SCIENCE, 2021, 42(9): 91-99.
[6]	YANG Chenglin, HUANG Chao, LIU Juan, HUANG Weimei, YUAN Zhihang, YI Jin’e, LIANG Zeng’enni, WU Jing. Protective Effect of Betulinic Acid on Liver Injury Induced by T-2 Toxin in Mice [J]. FOOD SCIENCE, 2021, 42(9): 107-114.
[7]	ZANG Yanqing, JU Xueying, ZHAI Yuqing, YAO Di, ZHU Lei, WANG Changyuan. Mechanism by which 10-Hydroxy-2-decenoic Acid Induces Cell Apoptosis and Cycle Arrest and Inhibits Migration in Hepatocellular Carcinoma HepG2 Cells [J]. FOOD SCIENCE, 2021, 42(23): 182-195.
[8]	HE Pingping, ZHENG Yajun, ZHENG Mingjing, JIANG Zedong, DU Xiping, ZHU Yanbing, NI Hui, LI Qingbiao. Protective Effect of Bangia fusco-purpurea Polysaccharide on H2O2-Induced Oxidative Damage in Caco-2 Cells [J]. FOOD SCIENCE, 2021, 42(17): 113-120.
[9]	YA Fuli, XIN Yu, ZHANG Chunmei, CHEN Binlin, LI Weiqi, MA Yongjie. Inhibition Effects and Mechanisms of Curcumin on Hydrogen Peroxide-Induced Platelet Apoptosis [J]. FOOD SCIENCE, 2021, 42(13): 151-157.
[10]	WANG Xinyi, ZHANG Yimin, LIANG Rongrong, DONG Pengcheng, LUO Xin, MAO Yanwei. Advances in Understanding the Regulation of Meat Tenderness by Peroxiredoxin 6 [J]. FOOD SCIENCE, 2021, 42(11): 236-243.
[11]	DU Yuemei, HAN Hedan, GUO Zhuoyu, GAO Liping. Effect of Grape Seed Proanthocyanidin Extract on Cisplatin-Induced Apoptosis of Mouse Testicular Sertoli TM4 Cells [J]. FOOD SCIENCE, 2020, 41(9): 98-104.
[12]	HAO Shuai, LI Shuang, WANG Jing, WU Tingting, ZHANG Jiawen, WANG Chengtao. RNA-seq Transcriptomic Analysis of Phycocyanin in Regulating the Inhibition of Non-small Cell Lung Cancer Cells [J]. FOOD SCIENCE, 2020, 41(5): 93-101.
[13]	GUO Yazhou, YANG Xiaoming, LI Yueying. Alkylresorcinols from Wheat Bran Induced Autophagy and Apoptosis of HepG2 Cells [J]. FOOD SCIENCE, 2020, 41(5): 102-107.
[14]	MA Ting, XIA Guanghua, LI Chuan, SHEN Xuanri. Inhibitory Effect of Tilapia Head Phospholipid on RANKL-Induced RAW264.7 Cell Differentiation and Mechanism of Osteoclast Apoptosis Induced by It [J]. FOOD SCIENCE, 2020, 41(3): 144-150.
[15]	TIAN Yan, DENG Fangming, ZHAO Lingyan, LIAO An, LAI Dengni, CHEN Qiujia. Inhibitory Effect of Glucosinolates from Brassica juncea var. integlifolia on HCT116 Human Colon Cancer Cells [J]. FOOD SCIENCE, 2020, 41(23): 172-180.

Protective Effect of Zeaxanthin on Tunicamycin-Induced Cell Cycle Arrest, Autophagy and Apoptosis of SH-SY5Y Cells

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments