食品科学 ›› 2011, Vol. 32 ›› Issue (22): 100-104.doi: 10.7506/spkx1002-6630-201122021

• 工艺技术 • 上一篇    下一篇

温州蜜柑皮多酚物质的超声提取及抗氧化特性

马亚琴1,叶兴乾2,吴厚玖1,周志钦1,3,王 华1,孙志高1   

  1. 1.西南大学柑桔研究所,国家柑桔工程技术研究中心 2.浙江大学生物系统工程与食品科学学院 3.西南大学园艺园林学院
  • 出版日期:2011-11-25 发布日期:2011-11-11
  • 基金资助:
    重庆市自然科学基金项目(cstc2011jjA80030);中央高校基本科研业务费专项(100030-2120130719)

Ultrasound-assisted Extraction of Polyphenols from Satsuma Mandarin (Citrus unshiu Marc) Peel and Its Antioxidant Capacity

MA Ya-qin1,YE Xing-qian2,WU Hou-jiu1,ZHOU Zhi-qin1,3,WANG Hua1,SUN Zhi-gao1   

  1. (1. Citrus Research Institute, Southwest University, National Citrus Engineering Research Center, Chongqing 400712, China; 2. College of Biosystem Engneering and Food Science, Zhejiang University, Hangzhou 310029, China; 3. College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400712, China)
  • Online:2011-11-25 Published:2011-11-11

摘要: 利用100kHz的超声波辅助提取温州蜜柑皮中的多酚类物质,并以传统的浸泡提取法作为对照与超声辅助提取法进行比较。结果表明,超声辅助提取法比浸泡提取法在低温条件下能更加快速、有效的提取酚酸。研究超声时间、提取温度、超声能量对酚酸含量及抗氧化能力的影响,发现温度对酚酸含量和稳定性的影响最为敏感,温度的增加能有效的提高酚酸含量,但是,当温度升高到40℃,延长超声时间造成酚酸含量的大幅度下降。此外,研究表明总酚含量和抗氧化能力呈现良好的线性关系,15、30、40℃处理后,相关系数R2依次为0.8435、0.8895、0.8622;在不同的超声能量水平下,R2为0.7971,这说明温州蜜柑皮是抗氧化物的丰富来源。研究表明超声辅助提取能有效的增加温州蜜柑皮提取物的酚酸含量和抗氧化能力,应用超声波技术增强温州蜜柑皮提取物的抗氧化能力具有潜在的优势。

关键词: 相关性, 超声辅助提取, 温州蜜柑皮, 多酚, 抗氧化特性

Abstract: Ultrasound at 100 kHz was used to enhance the extraction of polyphenolic compounds from Satsuma mandarin peel by the conventional soaking method. It was found that the addition of ultrasound assistance during the soaking of Satsuma mandarin peel in 80% methanol aqueous solution allowed more effective and efficient extraction of phenolic acids at low temperature. The effects of ultrasound treatment time, extraction temperature and ultrasound power on phenolic acid content and antioxidant capacity of extracts were studied and the results indicated that extraction temperature had the most important effect. A considerable increase of phenolic acid content in Satsuma mandarin peel extracts was observed with increasing temperature, while prolonged ultrasound treatment time resulted in a significant reduction of phenolic acid content at 40 ℃. Moreover, an excellent linear relationship between total phenolic content and antioxidant capacity of Satsuma mandarin peel extracts was found, revealing a R2 of 0.8435, 0.8895, 0.8622 at 15, 30 ℃ and 40 ℃ and of 0.7971 at various ultrasound power levels, respectively. Accordingly, Satsuma mandarin peel is an abundant source of antixodiants. This study also demonstrates that ultrasound assistance can effectively increase phenolic acid content and antioxidant capacity of Satsuma mandarin peel extracts.

Key words: ultrasound-assisted extraction, Satsuma mandarin peel, polyphenol, antioxidant capacity, relationship

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