In this study, a phylogenetic tree was constructed using bovine heat shock protein A6 (HSPA6) sequences and those of other organisms, and bioinformatic methods were used to analyze the basic physicochemical properties and hydrophilicity of bovine HSPA6 protein. Meanwhile, a protein interaction network was constructed to investigate the structural and functional properties of the protein encoded by the HSPA6 gene. The results showed that bovine HSPA6 protein had high similarity in amino acid sequence with those of sheep, Yangtze finless porpoise and other mammals. The molecular mass of bovine HSPA6 protein was 70 570.64 u, the theoretical isoelectric point was 5.66, and it was an acidic hydrophilic protein without transmembrane structure or signal peptide. Bovine HSPA6 protein may have 11 phosphorylation sites with score greater than 0.900 and N-glycosylation sites at the terminal bases, and it was a relatively stable protein with a secondary structure consisting mainly of 40.38% α-helix and 33.65% random coil, containing two major structural domains, the N-terminal nucleotide-binding domain and the C-terminal peptide-binding domain, which played a role in the cytoplasm. The constructed protein network showed that bovine HSPA6 protein mainly interacted with BAG1, DNAJA4, DNAJB1 and DNAJC2, and was involved in the activity of adenylate exchange factors, ATPase activity and chaperone binding, indicating that the HSPA6 protein exerted a potential function in biological processes such as energy metabolism in the bovine organism. These multiple bioinformatic analyses provide a theoretical basis for an in-depth investigation of the mechanism of the effect of bovine HSPA6 protein on meat quality.

Principal component analysis (PCA) and multiple linear regression analysis (MLRA) were used to analyze and investigate the suitability of green tea for tea tree varieties in different tea cultivars. The fresh leaves of 63 tea cultivars planted in the same garden were processed into roasted green tea by the same method. The sensory and major physicochemical qualities (tea polyphenols, free amino acids, water extracts and chlorophyll) and the color (L*, a* and b*) of green tea were analyzed. To evaluate the suitability of the tested cultivars for green tea processing and its major influential factors, the data obtained were analyzed by PCA and MLRA. The results showed that the coefficient of variation (CV) of chlorophyll content in different cultivars was the highest (26.7%), followed by tea a* value (22.2%), sensory score for tea infusion color (17.1%) and sensory score for tea color (16.2%). A significant correlation was found between the polyphenol content of green tea and its color, tea infusion color and the color of infused tea leaves; between the chlorophyll content, |a*| and b* values of green tea and its color; between the chlorophyll content and |a*| value of green tea and the color of infused tea leaves; and between the |a*| and b* values of green tea and tea infusion color (P < 0.01). The PCA results showed that the contribution rate of the first five principal components (PCs) was 76.895%, and that of the first principal component was 31.918%, mainly pointing to the color quality. According to the evaluation model constructed based on the first five PCs, the top 10 cultivars were Zhongcha 108, Wuniuzao, Pingyangtezao, Mengshan 9, Soubeizhong, Fudingdabai, Jiukeng 16, Fudingdahao, Maolv, and Longjing 43. Using MLRA, the regression function between overall sensory score (Y) and |a*| value (x) was obtained as Y = 68.668 + 5.174x (R2 = 0.313) (P < 0.001). The top 10 varieties determined from this equation were highly consistent with the results of PCA, indicating that a* value is an important indicator for the evaluation of the suitability of tea cultivars for green tea processing.

In order to uncover the role of the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) (PI3K/AKT) signaling pathway in metabolic regulation in Tan sheep meat during chilled storage, the transcriptome changes of lamb meat injected with the PI3K inhibitor LY294002 after 0, 4 and 8 days of chilled storage were studied by high-throughput sequencing. Altogether, 474, 96, and 13 differentially expressed genes (DEGs) between the control and LY294002 treatment groups were identified on days 0, 4, and 8, respectively. Functional enrichment analysis showed that the DEGs were mainly related to metabolic homeostasis, such as amino acid biosynthesis, glycolysis/gluconeogenesis, and glyceride metabolism. In addition, tissue section analysis showed that the gap between muscle fibers in the LY294002 group increased and the diameter of muscle fibers decreased significantly relative to the control group, indicating muscle atrophy. These results indicated that the PI3K/AKT signaling pathway played a crucial role in maintaining metabolic balance during the storage of Tan mutton, which in turn ensured the normal growth of muscle. This study provides a new direction for further elucidating the molecular mechanism by which the PI3K/AKT signaling regulates metabolic homeostasis in Tan mutton during chilled storage.

To investigate the inhibitory mechanism of small peptides on carbohydrate digestion, α-amylase and α-glucosidase inhibitory fractions from the water extract and the gastropancreatic digest of dry-cured ham muscle of Wanzhe spotted pigs were separated, purified, identified, and screened for peptide sequences. And the quantum chemical calculation was used to calculate structural and charge parameters including the distribution and energy of the frontier orbitals, electrostatic charge distribution and bond length, in order to speculate the active sites. It was found that the particle size of ham muscle decreased and its hypoglycemic activity increased after proteolysis. Two (S-I and S-II) and three fractions (WY-I, WY-II and WY-III) were obtained from the water extract and the digest after Sephadex column chromatography, respectively. Using mass spectrometry, 104 peptide sequences consisting of 8–24 amino acids were identified from fraction WY-II and five sequences with Peptide Ranker scores greater than 0.7 were selected. The highest occupied orbitals of the five sequences were mainly distributed in the guanidine groups of arginine and the groups close to the amino-terminal end, while the lowest unoccupied orbitals were in the carboxyl terminals and nearby groups. Sequences with lower ΔEL-H values, GPMGPSGPR, LGFGGPSGPNAGR and APAPAPAPAPPK, might be more active. According to Coulomb’s law, the active sites of these three peptides were located at –C106H108 of arginine, –C10H12 of leucine and –C176H177 of lysine, respectively. This study could provide theoretical support for understanding the blood glucose-regulating mechanism of peptides and the nutritional value of local pig breeds.

Objective: This study was conducted to investigate the effect of Enteromorpha prolifera polysaccharide (EP) on lipopolysaccharide (LPS)-induced intestinal barrier injury and the intestinal microbial composition in mice. Methods: Forty-eight 8-week-old C57BL/6J male mice were selected and randomly divided into four groups (12 mice/group): control (Ctrl), LPS, EP, and EP + LPS. The mice in the Ctrl and LPS groups were provided with a basal diet, and those in the EP and EP + LPS groups were given a basal diet supplemented with 600 mg/kg EP. After feeding for 28 days, the mice in the LPS and EP + LPS groups were injected intraperitoneally with 200 µL of LPS solution at a dose of 0.5 mg/kg body mass, and those in the Ctrl and EP groups were given the same dose of normal saline. Results: After 4 weeks of EP treatment, there was no significant change in LPS-induced liver damage or splenomegaly in mice (P > 0.05). Compared with the LPS group, EP treatment significantly reduced the serum levels of myeloperoxidase (MPO) (P < 0.05) and diamine oxidase (DAO) (P < 0.01), elevated the colonic gene expression of interleukin-1β (IL-1β) (P < 0.05), and decreased the colonic gene expression of inducible nitric oxide synthase (iNOS) (P < 0.05) and colonic Toll-like receptor 4 (TLR4) expression (P < 0.05). At the phylum level, EP treatment significantly increased the abundance of Verrucomicrobiota in mice with LPS-induced intestinal barrier injury (P < 0.01); at the genus level, it significantly decreased the abundance of Alloprevotella, Bacteroides and unclassified_o_Bacteroidales (P < 0.05) and increased the abundance of Lachnospiraceae_NK4A136_group, Anaerostipes and Akkermansia (P < 0.05). Conclusion: EP not only can prevent LPS-induced impairment of intestinal barrier function, but also can alleviate intestinal barrier damage in mice by regulating the expression of genes related to the TLR4 signaling pathway and the composition of the intestinal microbiota.

Objective: To explore the repairing effect and underlying mechanism of fucoxanthin on non-alcoholic fatty liver disease (NAFLD) induced by a high-fat diet (HFD) in C57BL/6 mice. Methods: Fifty-two C57BL/6 mice were randomly divided into four groups, including one normal group (n = 14) and three experimental groups (n = 38). The normal group was fed a regular diet, and the experimental groups were fed a HFD. After feeding for eight weeks, two animals were selected from the experimental groups for serum biochemical assays and liver histological observation, and the other 36 were divided into three groups (n = 12 each): model, low-dose and high-dose fucoxanthin, which were then administrated with physiological saline or fucoxanthin by gavage once a day for six weeks. Body mass was recorded weekly, and all mice were killed after fasting for 12 h at the end of the 14th week. The serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), free fatty acid (FFA), adiponectin, and leptin were measured. In addition, the levels of superoxide dismutase (SOD) activity, glutathione (GSH-Px), malondialdehyde (MDA), catalase (CAT), interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in liver homogenate were also determined. Furthermore, hepatic histopathological changes were observed under microscope, and the protein expressions of the adenosine 5’-monophosphate-activated protein kinase (AMPK), nuclear factor erythroid 2-related factor 2 (Nrf2) and toll-like receptors 4 (TLR4) signaling pathways in liver tissues were detected by Western blot. Results: Compared with the model group, the levels of TC, TG, LDL-C, ALT and AST in the fucoxanthin-treated groups were significantly decreased (P < 0.05), Leptin was decreased, while the levels of HDL-C and adiponectin were significantly increased (P < 0.05). Moreover, the levels of GSH-Px, SOD and CAT in the fucoxanthin-treated groups were significantly increased (P < 0.05), leptin was decreased, while the levels of MDA and inflammatory cytokines were significantly decreased (P < 0.05) compared with the model group. The results of hematoxylin-eosin (H&E) staining, oil red O staining, periodic acid-schiff staining (PAS), and transmission electron microscopy (TEM) showed that the histological structure of the liver in the fucoxanthin-treated groups recovered to almost normal. The results of Western blot showed that fucoxanthin treatment upregulated the protein expression of phosphorylated adenosine 5’-monophosphate-activated protein kinase (p-AMPK), peroxisome proliferators-activated receptor α (PPARα), phosphorylated acetyl-CoA carboxylase (p-ACC), and carnitine acyl transferase 1 (CPT-1) in the AMPK signaling pathway, downregulated the expression of sterol regulatory element binding protein-1c (SREBP-1c) and fatty acid synthase (FAS), inhibited the level of Kelch-like epichlorohydrin-associated protein-1 (Keap-1) in the Keap-1/nuclear factor-erythroid 2-related factor 2 (Nrf2) signaling pathway, increased the expression of Nrf2 and its downstream antioxidant proteins heme oxygenase-1 (HO-1), NAD(P)H quinone oxidoreductase 1 (NQO1), and glutamate cysteine ligase modifier (GCLM), and downregulated the expression of TLR4, myeloid differentiation factor 88 (MyD88), phosphorylated nuclear factor κB inhibitory protein α (p-IκBα), and phosphorylated nuclear factor κB (p65) (p-NF-κB (p65)) in the TLR4 signaling pathway. Conclusion: Fucoxanthin can repair HFD-induced NAFLD in mice through regulating lipid metabolism, reducing oxidative stress and suppressing inflammation.

Objective: To investigate the protective effect of garlic polysaccharides against metabolic-associated fatty liver disease (MAFLD) in mice. Methods: Totally 50 male C57BL/6 mice were randomly equally divided into five groups, namely, normal, model, low-, medium-, and high-dose garlic polysaccharides (250, 1 000, and 3 000 mg/kg mb, respectively). After 28 days of feeding, serum and liver samples were taken for pathomorphological examination, biochemical assays, and quantitative analysis of the expression of the genes Hmox1, Cat, Gpx1, Tnf, Ccl2, Cxcl2, Cxcl10, IL-1a, IL-1b, Fasn, Acaca, Cpt1a, Acox, Cd36, Fabp1, Apob and Mttp. Results: Compared with the model group, garlic polysaccharides at the medium and high doses significantly alleviated MAFLD, restoring the pathological morphology to normal, bringing the levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in liver tissue back to near-normal levels, and obviously restoring the expression of Hmox1, Tnf, Ccl2, Cxcl2, Cxcl10, Cpt1a, and Acox to normal levels. Conclusion: Intervention of garlic polysaccharides, especially at the medium dose, can basically eliminate liver damage caused by MAFLD. The underlying mechanism may be related to the regulation of lipid metabolism and inflammatory gene expression through suppressing the nuclear factor-κB signaling pathway, thereby reducing lipid deposition and lipid peroxidation.

This study aimed to construct a dose-response model of aflatoxin B1 (AFB1)-induced apoptosis rate in rat hepatocytes by using quantitative data. The coefficients of determination (R2) and further mathematical tests showed that the Gamma model (R2 = 0.996 4, Akaike information criterion = 37.40, and Bayesian information criterion = 17.19) was superior to other models. Hence, the Gamma model was recommended as the optimal dose-response model for apoptosis rate in rat hepatocytes after acute exposure to AFB1 for hazard characterization in the framework of AFB1 risk assessment.

The crude polyphenols extracted from Sargassum horneri were purified using macroporous adsorption resin, and the in vitro anti-inflammatory and hypoglycemic activities of the purified product were studied. Lipopolysaccharide (LPS) was used to induce RAW 264.7 macrophages to establish an in vitro inflammatory model. The optimal mass concentration of polyphenols from S. horneri was 40 μg/mL for reducing the NO content of inflammatory mediators. Compared with the model group, S. horneri polyphenols solution at 30 μg/mL significantly down-regulated the mRNA expression levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and IL-1β, and significantly inhibited the protein expression level of TNF-α, but had no significant inhibitory effect on the protein expression level of IL-1β; the inhibitory effect was positively correlated with the concentration of polyphenols. The half maximal inhibitory concentration (IC50) of S. horneri polyphenols on α-glucosidase was 5.96 μg/mL. According to the double reciprocal curve, the competitive inhibition constant (Kic) and noncompetitive inhibition constant (Kiu) were 0.06 and 6.68 μg/mL, respectively, indicating that the mode of inhibition was mixed-type with non-competitive being greater than competitive inhibition. Polyphenols from S. horneri have good anti-inflammatory and hypoglycemic activities, and can be used as a natural raw material in the fields of foods, health products and cosmetics.

The aim of this study was to explore the effect of Acer truncatum seed oil (ATO) on physiological indicators and the intestinal flora in aging Drosophila melanogaster. Wild-type D. melanogaster was cultured on a basal medium supplemented with 10, 20 or 40 g/kg ATO, and its behavioral changes such as lifespan, crawling ability, and olfactory memory and heat tolerance capacity were evaluated. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured. The midguts were collected to examine changes in gut microbiota composition by using 16S rRNA gene sequencing. Compared with the control group, the average lifespan of D. melanogasters in each ATO intervention group was significantly increased (P < 0.000 1); the most pronounced antiaging effect was observed at 20 g/kg ATO, where the average lifespan was prolonged by 31%. In addition, the crawling ability, olfactory memory and heat tolerance capacity were improved, and the activities of SOD and GSH-Px were increased in the 20 g/kg ATO intervention group. Aging changed the composition and structure of the gut microbiota in D. melanogasters. The results of linear discriminant analysis effect size (LEfSe) analysis suggested that the relative abundance of Enterobacteriaceae, Gluconobacter and Morganella morganii were increased in aging D. melanogasters, which was inhibited by ATO supplementation. To summarize, ATO has an anti-aging effect, alleviates physiological decline in D. melanogaster and prolongs its lifespan. The underlying mechanism may be related to changing the richness, evenness and structure of the gut microbiota in aging D. melanogasters, thereby regulating the gut microbiota and improving the antioxidant capacity.

In this study, the antihypertensive effect of microcapsules containing a mixture of goji berry, hawthorn and cassia seed at a mass ratio of 4:1:1 and angiotensin converting enzyme (ACE) inhibitory peptide in spontaneously hypertensive rats (SHR) was assessed. The ACE inhibition rate of the microcapsules containing the mixture and ACE inhibitory peptide at a mass ratio of 1:2 was 78.19%, which was significantly higher than those of the mixture of medicinal and culinary plant materials (69.46%) and ACE inhibitory peptide (71.48%) alone (P < 0.05). Under optimized encapsulation conditions (solid content of 10%, inlet air temperature of 160 ℃, core-to-wall ratio of 1:15, and a 1:4 mixture of modified starch and maltodextrin as wall material), an encapsulation efficiency of 82.76% was obtained. After intestinal digestion for 240 min, the release rate of the as-prepared microcapsules was 81.40%, and the bitter taste was significantly reduced (P < 0.05). Animal experiments showed that the microcapsules significantly reduced blood pressure in SHR, significantly increased the plasma contents of ACE2 and angiotensin 1-7 (Ang(1-7)), and reduced plasma ACE content and the contents of angiotensin II (AngII) in the plasma, heart, kidney and thoracic aorta (P < 0.05). The effect was more pronounced than those of the mixture of medicinal and culinary plant materials and ACE inhibitory peptide, confirming the synergism between them. The composite microcapsules can not only reduce blood pressure by regulating the renin-angiotensin system but also significantly reduce the degree of cardiac and thoracic aorta fibrosis in SHR, thereby improving organ damage caused by hypertension.

Objective: To comprehensively evaluate the hypoglycemic and antioxidant effects of Camellia nitidissima flower. Methods: The chemical constituents in the aqueous extract of C. nitidissima flower (CFA) were identified by high performance liquid chromatography-mass spectrometry (HPLC-MS). Next, a mouse model of type 2 diabetes was established, and the diabetic mice were randomly divided into five groups: model, positive control (acarbose at 20 mg/kg mb), low-, medium- and high-dose CFA (200, 400 and 800 mg/kg mb, respectively). After five weeks of intragastric intervention, general growth characteristics, serum glucose, fasting insulin (FINS) and lipid levels, oxidative stress in pancreas and liver tissues, tissue morphological changes and cell apoptosis were analyzed. Results: CFA had a high content of polyphenols and polysaccharides. The half-maximal inhibitory concentration (IC50) values for scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and α-glucosidase inhibition were (24.14 ± 0.64) and (69.99 ± 1.97) μg/mL, respectively. Seven compounds were identified from CFA. In addition, CFA could effectively improve the ‘three more and one less’ symptoms of diabetic mice, significantly reduce the levels of fasting blood glucose (FBG), postprandial blood glucose (PBG), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and malondialdehyde (MDA), increase the levels of insulin and high-density lipoprotein cholesterol (HDL-C), improve the activities of total superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and alleviate pathological damage in pancreas and liver tissues. Conclusion: CFA has significant hypoglycemic and antioxidant effects on type 2 diabetic mice.

In order to investigate the effect of low-temperature air cooling on the postmortem energy metabolism and meat quality of yellow-feathered broilers during mixed cooling (first water cooling and then air cooling), 85-day-old broilers were slaughtered and divided into three groups: treatment (water cooling at 0–4 ℃ followed by air cooling at −8, −18, −25 or −31 ℃), control (traditional water cooling at 0–4 ℃) and traditional mixed cooling (water cooling at 0–4 ℃ followed by air cooling at 0–4 ℃) groups. Finally, all carcasses were cooled to an internal temperature of 4 ℃, and the optimal air cooling temperature was determined by measuring cooling rate, pH, energy metabolism and quality indexes. The results showed that the cooling time of air cooling at −25 and −31 ℃ was 2 537 and 2 272 s, respectively, which was shortened by 27.78% and 35.33% compared with the traditional water cooling and by 51.87% and 56.90% compared with the traditional mixed cooling, respectively. Air cooling at −25 and −31 ℃ delayed the decrease in pH, reduced the drip loss and cooking loss of broiler breast muscle, significantly increased the shear force and the content of immobilized water compared with the control group (P < 0.05). Compared with the other groups, the activities of muscle hexokinases, phosphate-3-phosphate dehydrogenase and lactate dehydrogenase in the air cooling treatments at −25 and −31 ℃ were lower, and the consumptions of adenosine triphosphate (ATP) and glycogen, the production of lactic acid and adenosine monophosphate (AMP) and AMP/ATP ratio were the smallest (P < 0.05). The above findings showed that air cooling at −25 and −31 ℃ significantly inhibited the rate of anaerobic glycolysis during the early postmortem period in chicken meat, delayed the rate of pH decline, and effectively improved muscle water retention. From the perspective of industrial energy saving, −25 ℃ is determined as the best temperature for air cooling treatment.

Magnetic fields can improve the quality of foods by influencing the arrangement of water molecules, thus altering the distribution of water in foods. In this study, dough was fermented at magnetic field strengths of 0, 0.5, 1.0, 1.5, and 2.0 mT for 1 h. The effect of magnetic field treatment on the fermentation characteristics, moisture distribution, and protein molecular structure of dough was investigated by low-field nuclear magnetic resonance (NMR) and in situ Raman spectroscopy, with a view to providing a theoretical basis for the development of high-quality Chinese dough-based foods. The results showed that the fermentability of dough tended to increase first and then decrease as the magnetic field strength increased, and the most significant effect on the fermentability was observed at a magnetic field strength of 1.5 mT. The specific volume of steamed bread initially increased and then decreased. When the magnetic field strength was 1.0 mT, the specific volume was 2.24 mL/g. The hardness, stickiness, and chewiness showed a trend of initially decreasing and then increasing, but there was no obvious change in the elasticity, cohesiveness or resilience. The contents of strongly bound water and free water initially decreased and then increased, whereas the opposite trend was observed for weakly bound water. The effect of 1.0 mT magnetic field strength on the water distribution was most significant. The relative content of α-helix increased first and then declined, while the reverse trend was observed for random coil. At a magnetic field strength of 0.5 mT, the relative content of α-helix was the highest (54%) and the relative content of random coil was lowest (21%). Proper magnetic field treatment could increase the fermentability of dough, the specific volume and textural properties of steamed bread, accelerate the water distribution in dough and improve the stability of the gluten network. In conclusion, magnetic field treatment could improve the fermentability of dough and the textural properties of steamed bread by improving the distribution of water, increasing the moisture uniformity, and changing the molecular structure of gluten proteins in dough.

In order to study the pattern of quality changes in selenium-rich rice during storage, selenium-rich rice and non-selenium-rich rice produced in Enshi and Jingzhou of Hubei province were subjected to accelerated aging for 180 days under high temperature storage conditions (35 ℃, and 50% relative humidity) in an artificial climate incubator. The storage characteristics, gelatinization characteristics and edible quality were measured and analyzed every 30 days. The results showed that with increasing storage time, the trends of changes in all tested indicators were consistent between selenium-rich rice and non-selenium-rich rice, but the degree of change was different. On day 180, the germination rate of non-selenium-rich rice was 69.7% and 65.7% for the cultivars Ezhong 6 and Daojingliangyou, the content of fatty acids increased by 40.43% and 59.74% compared with those on day 0, and α-amylase activity was 0.26 and 0.22 U/g, respectively. For these two cultivars, the germination rate of selenium-rich rice was 66.7% and 64.0%, and the content of fatty acids increased by 53.99% and 78.47% compared with those on day 0, and α-amylase activity was 0.24 and 0.19 U/g, respectively. Compared with the control group, selenium-rich rice had lower germination rate, lower α-amylase activity and higher fatty acid value, indicating no obvious advantages in storage quality. On day 180, the content of malondialdehyde (MDA) was 0.36 and 0.28 μmol/g in ordinary Ezhong 6 and Daojingliangyou rice, the content of free phenols was 341.78 and 371.59 μg/g, and free sulfhydryl group content was 0.67 and 0.64 μmol/g, respectively; for selenium-enriched Ezhong 6 and Daojingliangyou rice, MDA content was 0.31 and 0.24 μmol/g, free phenol content was 368.33 and 399.22 μg/g, and free sulfhydryl group content was 0.89 and 0.74 μmol/g, respectively. Compared with the control group, selenium-rich rice had lower MDA content, and higher contents of free phenols and free sulfhydryl groups, indicating better antioxidant capacity during storage. In terms of gelatinization characteristics, texture characteristics and edible quality, compared with the control group, selenium-rich rice had higher peak viscosity, lower gelatinization temperature, lower hardness, higher viscosity, higher elasticity and higher taste score during the same storage period. In conclusion, selenium-rich rice had better antioxidant capacity and higher taste score during storage at 35 ℃, but did not show anti-aging advantages.

In this study, a nano-Cu/polysaccharide composite film was prepared by the solution casting method using gelatin and sodium alginate as film-forming substrates. Before casting, green synthesized nano-Cu was incorporated into the film-forming solution by co-blending method. Field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TAG), diffuse reflectance spectroscopy (DRS), texture analysis (TA) and inductively coupled plasma-mass spectrometry (ICP-MS) were used to characterize the structure, light transmittance and physicochemical properties of nano-Cu and the composite film. The antifungal activity of the composite film was also evaluated and applied to the biological control of the black spot disease of winter jujube. Finally, the migration of Cu2+ in the composite film was measured. The results showed that the particle size of green synthetic nano-Cu was approximately 44 nm, and gelatin/sodium alginate could be used as an excellent carrier for nano-Cu. The composite film had good thermal stability, barrier properties and mechanical properties. In addition, the inhibition rates of the composite films with different concentrations of nano-Cu against Alternaria alternata, Fusarium and Botrytis cinerea were up to 87.80%, 77.73% and 81.96%, respectively, showing good and broad-spectrum antifungal properties. The half maximal inhibitory concentration (IC50) of nano-Cu against A. alternata biomass was 0.25 g/L. When stored for 10 days, the composite film with nano-Cu at 0.25 g/L reduced the lesion diameter by 52.53% and the incidence of black spot disease by 53.16% compared with the control group, and the migration of Cu2+ was 0.018 7 μg/mL. This study provides a new idea for the application of nano-Cu and a theoretical basis for the development of new antifungal materials.

Using corn starch (CS) as the film substrate, citric acid as the crosslinking agent, and Pickering emulsion prepared with cellulose nanocrystals (CNC)-emulsified orange peel essential oil (OPO) as the antibacterial agent, an antibacterial composite film (CS/CNC-OPO) was successfully prepared by the casting method. The effects of different contents of CNC and OPO on the mechanical strength, water vapor transmission rate, water contact angle and antibacterial properties of the composite film were analyzed, and the composite film was characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) spectroscopy and scanning electron microscopy (SEM). The results showed that the performance of the CS/CNC-OPO antibacterial film was the best when 11% OPO was added and the CNC-to-OPO ratio was 1:11 (g/mL). The antibacterial film had the following properties: water vapor permeability, 1.03 × 10-3 g/(m·h·kPa); water contact angle, 74.53°; and tensile strength 6.01 MPa. The area of inhibition zone of the film against Escherichia coli and Staphylococcus aureus was up to 88.4 and 96.45 mm2, respectively. Therefore, the starch-based film has good strength, hydrophobic and antibacterial properties, and has broad application prospects in the field of food packaging.

In order to investigate the inhibitory effect of ε-poly-L-lysine (ε-PL) on the occurrence of Lasiodiplodia theobromae-induced diseases and its correlation with disease resistance in postharvest passion fruits, harvested golden passion fruits (cv. Fujian Baixiangguo 3) were immersed in either sterile distilled water (as control group) or 100 mg/L ε-PL solution for 10 min, inoculated with L. theobromae, and stored at (28 ± 1) ℃ and 90% relative humidity for up to 7 days. The changes of fruit lesion diameter, cell membrane permeability, lignin content and disease resistance-related enzyme activities in the pericarp were assayed every day. The results showed that compared to the control group, ε-PL delayed the increase in cell membrane permeability and lesion diameter, and enhanced the content of lignin and the activities of disease resistance-related enzymes including cinnamate-4-hydroxylase (C4H), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), peroxidase (POD), β-1,3-glucanase (GLU), 4-coumarate CoA ligase (4-CL), chitinase (CHI) and cinnamyl alcohol dehydrogenase (CAD), thereby maintaining cell membrane integrity by delaying the increase of cell membrane permeability, enhancing disease resistance, and inhibiting the occurrence of L. theobromae-induced diseases in harvested passion fruits.

The objective of this study was to explore the effect of the vent mode of corrugated boxes universally used in the market on the precooling performance of layered peaches and to determine the functional relationship between the precooling environmental parameters and the precooling efficiency and the optimal vent mode under different differential pressure pre-cooling working conditions in order to realize the rapid energy-saving precooling of peaches after harvest. A numerical model of heat and mass transfer during differential pressure precooling with circular and rectangular vents (abbreviated as CV and RV, respectively) was established based on computational fluid dynamics. By comparing and analyzing the experimental and simulated data, it was found that the maximum root mean square error and mean absolute percentage error between the two vent designs were 0.799 ℃ and 6.6%, respectively, which fully verified that this numerical model had high prediction accuracy. Through in-depth exploration of the temperature and flow field distribution in different vent modes, it was found that CV exhibited inferior precooling uniformity when compared with RV. Nevertheless, CV demonstrated a notable reduction in precooling time by 30%–40% and a decrease in fan energy consumption by 50%. Additionally, their relationships with differential pressure were described by. Based on these obtained results, the precooling quality of peaches could be improved by using RV, and the precooling cost could be reduced by using CV. To simultaneously achieve these two goals, the diameter of CV should be greater than 35 mm. This study provides a theoretical reference for the reasonable selection of vent parameters and accurate monitoring of fruit precooling performance in small and medium-sized orchards.

In order to prolong the storage period of ‘Huangguan’ pear and reduce the deterioration of fruit quality during long-term storage and subsequent shelf life, this study conducted preharvest treatment with different concentrations of aminoethoxyvinylglycine (AVG) to ‘Huangguan’ pear. Before and after refrigeration for 180 days and after subsequent shelf life for 7 days, quality indicators were tested. The results showed that compared with the control group, preharvest AVG treatment delayed the decline of fruit firmness, maintained higher contents of soluble solids and titratable acids, and effectively inhibited fruit surface browning and core browning, the most pronounced effect being observed at 200 mg/L AVG. During long-term cold storage, the contents of arbutin, chlorogenic acid and total phenols increased with the occurrence of core browning, and the activity of polyphenol oxidase (PPO) increased. The expression levels of PbPAL1, PbPAL2, PbPPO1, PbPPO5, PbLOX1 and PbLOX5 were up-regulated during cold storage, but down-regulated during shelf life. Preharvest treatment with 200 mg/L AVG significantly reduced the consumption of arbutin, chlorogenic acid and total phenols, inhibited the increase of PPO activity along with the expression of PbPAL1, PbPAL2, PbPPO1, PbPPO5 and PbLOX5, and thus effectively reduced core browning in ‘Huangguan’ pear .

In order to investigate the effect of Bacillus amyloliquefaciens GSBa-1 on polyphenol synthesis and phenylpropanoid metabolism in Citrus medica L. var. sarcodactylis Swingle stored at normal temperature, C. medica L. var. sarcodactylis Swingle was soaked in a 1 × 108 CFU/mL suspension of B. amyloliquefaciens GSBa-1, taken out and air-dried before being stored at room temperature. The contents of total soluble solids (TSS) and titratable acids (TA), ethylene release, respiration intensity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, H2O2 concentration, polyphenol content and enzyme activities related to the phenylpropane metabolic pathway were measured. The results showed that treatment with GSBa-1 reduced ethylene release and respiratory intensity, inhibited peel color change and fruit softening, induced an increase in phenylalanine ammonia lyase (PAL) and cinnamate-4-hydroxylase (C4H) activities, rapidly promoted the synthesis of gallic acid and caffeic acid as representatives of phenolic acids, induced the accumulation of flavonoids and flavanols such as hesperidin, seroxin and diosmin, increased the contents of total phenols and total flavonoids, and improved DPPH radical scavenging capacity and H2O2 content and consequently fruit resistance. It can be concluded that B. amylolytic GSBa-1 can activate the phenylpropane metabolic pathway, and stimulate the synthesis and accumulation of polyphenols, thereby delaying post-ripening and senescence and improving storage quality of C. medica L. var. sarcodactylis Swingle.

In view of the drawback of traditional deterministic prediction that it cannot provide uncertainty information, this study proposed a prediction model that integrates point estimation and interval estimation, and innovatively applied it to the field of food safety risk pre-warning. In the point estimation, wavelet packet decomposition (WPD) was used to decompose the weekly risk level sequence and the autoregressive integrated moving average (ARIMA) model was used for prediction. In the interval estimation, the generalized autoregressive conditional heteroskedastic (GARCH) model was used to predict the residual. The WPD-ARIMA-GARCH model established in this study was applied to the safety risk prediction of soy sauce and pot-roast meat products from a certain region. The results showed that the safety risk of soy sauce and pot-roast meat products from this region was relatively high at the end of March and July in 2019, which was consistent with the actual situation. Meanwhile, in the risk prediction of soy sauce and pot-roast meat products from 10 different regions, the mean square error, mean absolute error, and mean absolute percentage error of the model were 1.626, 0.806, and 20.824, respectively, and the prediction interval normalized average and coverage width-based criterion values at the 90% confidence interval were both 0.024, which could cover all true values. Therefore, the model has high prediction accuracy and low error, is useful for risk control for the quality and safety of soy sauce and pot-roast meat products, and provide technical support for daily food safety supervision.

An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of the residues of prometryn and its metabolites in aquatic products was developed using a modified quick, easy, cheap, effective, rugged and safe (QuEChERS) method for sample pretreatment. Homogenized samples were extracted with acetonitrile and purified by the combined use of enhanced matrix removal of lipids (EMR-Lipid) and Cleanert LipoNo. The detection was carried out in the positive ion mode with selected reaction monitoring (SRM) and the internal standard method was selected for quantification. Under the optimal conditions, the calibration curves for prometryn and its four metabolites were linear with correlation coefficient (R2) above 0.992 in the concentration range of 1–500 ng/mL. The limits of detection (LODs) and limits of quantitation (LOQs) for all analytes were as low as 0.20 and 0.50 μg/kg, respectively. For spiked grass carp, crayfish and Chinese mitten crab, good accuracy and precision were observed, with average recoveries and relative standard deviations (RSDs) ranging from 74.4% to 113.7% and 3.17% to 11.47%, respectively. After correction by the internal standard method, the matrix effects of the five compounds in different aquatic products were not significant. The developed method allows the identification and quantitative analysis of the residues of prometryn and its metabolites in aquatic products. Additionally, the applicability of this method was validated by detecting positive samples of crayfish bathed with prometryn.

In order to explore quality evaluation methods for Chinese coffee beans for freshly ground coffee and instant coffee and to select evaluation indicators, the major quality indicators were selected from the basic quality indicators through the Delphi evaluation method and sequence relationship analysis to construct a quality index system. Then the differences in the major quality indicators of 53 coffee bean samples from different production areas in Yunnan and Hainan provinces were determined, the characteristic quality indicators of coffee beans for different purposes were selected by correlation analysis, principal component analysis (PCA) and cluster analysis, and stepwise multiple linear regression analysis was conducted based on the comprehensive scores of the principal components of the samples. Finally, grain size, taste, aroma and carbohydrate content were selected as the characteristic quality indicators of instant coffee and grain size, taste, aroma and caffeine content as the characteristic quality indicators of freshly ground coffee. It is determined that the major varieties suitable for instant coffee are Catimor and Typica, and the major production area is Yunnan province. The major variety suitable for freshly ground coffee is Catimor, and the major production area is Yunnan province.

Objective: A recombinase-mediated strand displacement isothermal amplification assay for rapid detection of horse-derived components in meat and meat products was established. Methods: A series of specific primers and Exo probes were designed using the horse-derived ATpase 6 gene as the target gene. The primers were screened and the reaction parameters were optimized. The specificity, sensitivity and stability of the assay were evaluated, and the detection limit, applicability and accuracy were analyzed by detecting simulated samples with different mixing ratios and different processing technologies, and commercial samples. Results: The assay was characterized by rapid response, high sensitivity and specificity. The reaction was completed within 16 minutes under a constant temperature of 39 ℃. The system had good specificity to 23 non-target sources. The detection sensitivity of the target DNA was 1.8 copies/μL. The detection limit was 0.01% for raw meat (mass fraction), and 0.1% (mass fraction) for processed meat products. For 90 commercial samples, the results of this method were consistent with those of the standard method. Conclusion: The recombinase-mediated isothermal amplification assay can be used for the detection of horse-derived components in meat and meat products.

In this study, 17 deoxynivalenol (DON) contaminated wheat samples were assessed for safety and quality index such as unit weight, water absorption of gluten, crude protein content, unsound kernel content, sprouted kernel content, gibberella damaged kernel content and insect infested kernel content as well as the pollution status of DON and heavy metals. Furthermore, the relationship between the content of DON in wheat grains and other parameters was analyzed. The impact of the processing of gluten and starch in the break and reduction systems on the level of DON contamination was examined. The results showed that the pollution level of DON in wheat samples had a good linear correlation with unit weight, water absorption of gluten, crude protein content, unsound kernel content, sprouted kernel content, gibberella damaged kernel content and insect infested kernel content. The correlation between gluten water absorption and the content of DON was not significant, indicating that DON content could not affect the storage quality of wheat. Additionally, it was found that the heavy metals As and Cd as well as the elements Ca and P were not significantly correlated with DON content, but only Ca in whole wheat flour was correlated with the content of DON. The levels of DON in gluten and starch from the break and reduction systems, respectively were both lower than 200 µg/kg, indicating that wheat with DON over-standard can be used commercially for the preparation of gluten or starch.

The widespread presence of foodborne pathogenic bacteria biofilms in the food supply chain has significantly negative impacts on food microbial safety, posing a serious threat to the health and safety of consumers. Weakening the interaction between food contact surfaces and biological pollutants and reducing biological adhesion by changing the physicochemical properties of food contact surfaces can effectively reduce the formation of biofilms, thereby ensuring the microbial safety of food. This article summarizes recent progress in research on anti-biofouling materials. First, the application of the DLVO theory to quantify the interaction between biological pollutants and material surfaces is reviewed. Then, this paper classifies the existing anti-biofouling materials into two categories: hydrophobic and hydrophilic, and summarizes the typical methods to construct each type of anti-biofouling materials and their anti-biofouling mechanisms. Finally, based on the current status of the application of anti-biofouling materials in the food industry, an outlook on future development directions is provided. This article aims to provide theoretical guidance for the development of new anti-biofouling materials in order to better ensure the microbial safety of food.

Emulsion is considered as a good system for delivering bioactive substances, but its instability limits its application. Protein-derived peptides have unique nutritional value and biological activity, possess a flexible structure, and are prone to unfolding and rearrangement at the oil-water interface, so emulsions stabilized by protein-derived peptides have received a lot of attention. Although protein-derived peptides can act as an effective emulsifier to form emulsions, they are inefficient in stabilizing the emulsions. Studies have found that emulsions stabilized with Maillard reaction products from protein-derived peptides have good stability, physicochemical and functional properties. Therefore, the action mechanism, physicochemical and functional properties of emulsions stabilized with Maillard reaction products from protein-derived peptides and the factors affecting their stability are elaborated in this review in order to provide a theoretical basis for expanding the application of protein-derived peptide stabilized emulsions in the delivery of bioactive substances.

Electrochemiluminescence (ECL) is a chemiluminescence phenomenon triggered by electrochemical techniques. It has the advantages of near-zero background, high sensitivity, wide detection range, and good controllability, which has been widely commercialized in the field of in vitro diagnosis. Therefore, ECL has become one of the current research hotspots in the fields of food safety and environmental monitoring. This article summarizes the mechanism and system composition of ECL, and focuses on the application of ECL combined with immunoassay, and aptamer or molecular imprinting technology in the detection of mycotoxins. Finally, the major problems and challenges in the application of ECL in food safety detection are proposed and analyzed.

A Pickering emulsion is an emulsion stabilized by solid particles. As most natural colloidal particles used for stabilizing Pickering emulsions have poor emulsifying performance, it is necessary to improve their emulsifying performance. At present, protein-based particles have been widely applied in food-grade Pickering emulsions, while polysaccharide-based particles have scarcely been researched. Compared with pure and mixed polysaccharide particles, non-covalent and covalent complexes between polyphenols and polysaccharides not only have excellent emulsifying performance, but also can improve the oxidation stability of Pickering emulsions and endow it with controllable rheological properties, and broad-spectrum antibacterial properties. However, there are very few systematic reviews on the application of polyphenol-polysaccharide complexes in food-grade Pickering emulsions. Therefore, beginning with an introduction to the stability mechanism of Pickering emulsions and the formation mechanism of polyphenol-polysaccharide complexes, this article reviews the application of polyphenol-polysaccharide complexes in food-grade Pickering emulsions to provide a reference for the application of polyphenol-polysaccharide complexes in food, medicine, cosmetics and other fields.

Three-dimensional (3D) printing, also known as additive manufacturing, is an emerging manufacturing technology that enables personalized product design and precise modeling through digital control. In recent years, 3D printing technology has gained significant attention in the food industry due to its potential advantages, especially in the field of customized food processing. Starch is an important component of human diet, especially in the eastern diet structure primarily based on plant-based foods. Most starches possess excellent rheological, hydration, and gel properties, making them have natural advantages in outflow nozzle and printing molding and thus have great application potential in food 3D printing. In this article, recent progress in starch-based 3D printing is reviewed with respect to printing equipment types commonly used in starch-based 3D printing, printing technology using common starches as raw materials, the correlation between starch physicochemical properties and printing performance, starch modification for quality improvement of 3D printed products, the post-processing of starch-based 3D printed products and the influence of printing on starch structure. Furthermore, future prospects in the 3D printing field are presented.

1,3-Diacylglycerol (DAG) has been well recognized as a safe food component. The metabolic pathway of DAG in the body is different from that of triacylglycerol, and DAG has been demonstrated to contribute to improving metabolism and preventing obesity and cardiovascular diseases. However, the mass fraction of natural DAG in edible oils and fats is less than 10%, so the preparation of high-purity DAG has received widespread attention in recent years. Enzymatic synthesis is the method of choice for the industrial preparation of DAG due to the advantages of eco-friendliness, safety and desirable selectivity over chemical synthesis. Edible livestock and poultry fats, as by-products of slaughter and processing, have a special flavor and nutritional value. However, their undesirable physicochemical properties, especially high melting points and high levels of saturated fatty acids and cholesterols, highly limit their applications in the food industry. In light of this, the synthesis of DAG from edible livestock and poultry fats is a promising approach for their high-value utilization. The current status of the enzymatic synthesis and the potential application of DAG from livestock and poultry fats are reviewed in this article. It is anticipated that this review will provide new ideas for researchers interested in the enzymatic preparation and high-value utilization of DAG from livestock and poultry fats.

Food traceability is an important means to ensure food quality and safety. For the purpose of providing a reference for future research on food traceability in China, a comprehensive bibliometric and visual analysis of papers concerning global food traceability included in the Science Citation Index Expanded (SCIE) database from 2001 to 2022 was performed. The results showed that 3 020 authors from 1 136 research institutions in 80 countries/regions published their research on food traceability in 255 journals, indicating frequent cross-national and cross-regional cooperation and cross-disciplinary integration. China, Italy, the United States, Spain, the Netherlands produced a large volume of research and built strong cooperation. The overall quality of food traceability research in Italy, the United States, the Netherlands, and Spain was high. The research fields were concentrated in food science and technology, chemistry, agriculture, and engineering. Additionally, many research achievements were made in the fields of computer science, ecology and environmental science, nutrition and dietetics. The research focuses included food quality and safety, food supply chain management, geographical origin, traceability technology, food authentication and certification, and willingness to pay. In the future, the academic frontier will focus on three aspects: intelligent food traceability system based on Industry 4.0, food authentication and detection technology represented by genomics and next-generation sequencing technology, and the mechanisms of the motivation of participants, interest game and premium payment in food traceability system.

Phytoglycogen (PG), a carbohydrate polymer extracted from sugar-containing mutant seeds of plants, is a natural dendrimer-like glucan nanoparticle with high safety. The highly branched structure of phytoglycogen that is externally compact and internally loose provides it with high water retention, low viscosity, good dispersion stability, strong antioxidant and antibacterial activity, film-forming capacity, and excellent load capacity. Thus, in recent years, phytoglycogen has gradually become one of the research hotspots. However, there are few reports on the extraction, structural analysis, and comprehensive utilization of phytoglycogen so far. Therefore, this review focuses on the structure and properties of phytoglycogen, discusses the latest extraction methods for phytoglycogen, summarizes its application in foods, and provides an outlook on future research prospects, aiming to provide a theoretical basis for expanding the application of phytoglycogen.

Electrochemical technology detects analytes based on their electrochemical signals. Due to its advantages of simple operation, low cost, high precision and sensitivity, the application of electrochemical technology in food detection and analysis has attracted much attention, and some research progress has been made. In this article, the types, basic principles and applications of electrochemical methods in the field of food detection are reviewed, and prospects for the application and development of electrochemical technology are discussed to provide a reference for promoting the application of electrochemical technology in the field of food detection and analysis in order to meet the development needs of rapid food detection and analysis.

Polyphenols, a class of compounds in plant foods, have excellent biological activities, including antioxidant, anti-inflammatory, and antitumor effects. However, polyphenols are susceptible to external adverse factors such as high temperature, light and alkaline conditions during food processing, storage, and digestion, resulting in partial or complete loss of their biological activities. As a natural polysaccharide, gel systems based on starch have good biocompatibility, degradability and safety, as well as excellent encapsulation capacity and effective controlled release effect on active small-molecule compounds due to their unique three-dimensional network structures. Starch-based gels fabricated through different approaches including 3D printing, covalent or non-covalent cross-linking, and gel fillers have a promising prospect in improving the stability and bioavailability of polyphenols. In this article, the biological activities and limitations of polyphenols are described. The relationship between starch properties such as gelatinization, retrogradation and gelling properties as well as different cross-linking strategies and the formation of starch-based gels is discussed. Furthermore, the role of polyphenols in the formation of starch-based gels and how starch-based gels in turn affect the encapsulation and release properties of polyphenols are analyzed. Finally, future applications and challenges of polyphenols encapsulated by starch-based gel systems in the food industry are also discussed.

Saccharomyces cerevisiae has been applied widely in industrial production; however, a variety of stressors and inhibitors limit its application. Therefore, exploring the tolerance mechanism of S. cerevisiae to various environmental adverse factors and improving its tolerance by various methods such as genetic modification are crucial for improving the robustness and fermentation ability of S. cerevisiae in industrial production. This article reviews the tolerance mechanism of S. cerevisiae to osmotic pressure, ethanol, high temperature, organic acid, reactive oxygen species (ROS) and sulfur dioxide, analyzes tolerance genes related to environmental stress in S. cerevisiae and summarizes the current directions in research on the tolerance mechanism of S. cerevisiae. In the future, the molecular mechanism and functional network of strain adaptation to the environment will be studied by systems biology and genetic engineering technology, which will provide theoretical and practical guidance for improving the stress resistance and fermentation ability of S. cerevisiae in industrial production.

With the gradual advancement of the Healthy China strategy, the comprehensive healthcare industry represented by “homology of medicine and food” has gradually become a hot topic. As one of the most consumed condiments in China, chili can be used for medicinal and culinary purposes. Capsaicin (CAP), a major bioactive substance in chili, possesses biological effects such as analgesic, antioxidant, anti-inflammatory, anti-cancer, anti-obesity and cardioprotective and has been extensively studied for its pharmacological functions and potential action mechanisms. Considering that the application of CAP has some problems including high irritation, poor water solubility and low bioavailability, this paper reviews the evaluation of novel delivery systems for improving the oral bioavailability of capsaicin, including liposomes, micelles, micro/nanoemulsions, Pickering emulsions and nanoparticles based on stability, encapsulation rate and biological properties. This review aims to provide a theoretical basis for the development of capsaicin into functional nutraceuticals.

As the country has loosened its family planning policy and living standards have improved, postnatal obesity has become a social problem seriously threatening public health in China, which has attracted widespread attention from all walks of life. Therefore, a method for improving postpartum obesity urgently needs to be explored. Intestinal microecology is critical to the health of mothers and children, and plays an important role in the occurrence and development of obesity. Improving intestinal microecology as an effective way to improve obesity has attracted more and more attention. This article focuses on the relationship between postnatal obesity and intestinal microecology and summarizes the mechanisms of action of probiotics and prebiotics in regulating intestinal microecology to suppress postpartum obesity. The purpose of this article is to provide a theoretical basis for probiotic-prebiotic intervention based on intestinal microecology to suppress postpartum obesity.

The contamination of foodborne pathogenic bacteria poses great threats to human health. Conventional foodborne pathogen detection methods suffer from the shortcomings of time-consuming, cumbersome, and non-real time. Hence, it is of great significance to explore a sensitive, safe, simple and economical method for foodborne pathogen detection. Compared with the traditional methods, nano-biosensor-based detection assays possess merits such as exceptional sensitivity, high selectivity, real-time detection, low consumption and low limit of detection. In this article, these nano-biosensor-based methods are summarized, and the principles and advantages of single-, dual- and multi-mode detection are comprehensively discussed. Meanwhile, an outlook on the application of nano-biosensors for the detection of foodborne pathogens is given with the goal of providing a reference for the optimization of the existing detection methods for foodborne pathogenic bacteria.