In this study, the optimal process conditions for supercritical CO2 extraction of oil from Ganoderma lucidum spore
and its volatile compounds were investigated, aiming to provide a theoretical basis for its identification, quality control and
further development. Single factor experiment and orthogonal array design were employed to obtain the optimal extraction
conditions with respect to oil yield and triterpenoid compounds yield; the volatile compounds in Ganoderma lucidum spore
oil were analyzed by headspace solid-phase microextration method combined with gas chromatography-mass spectrometry.
The optimal extraction and separation conditions were determined as the follows: extraction pressure, 30 MPa; extraction
temperature, 40 ℃; separation pressure, 8 MPa, and separation temperature, 56 ℃. Under these conditions, the oil yield
and triterpenoid compounds yield were 29.45%, and 38.14 g/kg, respectively. A total of 65 volatile compounds, accounting
for 83.87% of the total volatile compounds, were isolated and identified from Ganoderma lucidum spore oil, among which
hydrocarbons, alcohols, aldehydes and esters were the major components. Terpenes, alcohols, aromatic hydrocarbons and
aldehydes were main odor compounds, among which benzyl alcohol (16.35%) and phenylethyl alcohol (5.74%) with the
highest relative proportion may be the characteristic volatile compounds.
