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15 July 2016, Volume 37 Issue 13
Basic Research
Stability of Polyphenols during in Vitro Digestion
LI Ti1, WANG Xieyi1, ZHAI Yuxin1, DAI Taotao1, CHEN Jun1,*, LIU Jiyan2, LIU Chengmei1
2016, 37(13):  1-5.  doi:10.7506/spkx1002-6630-201613001
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The aims of this study were to investigate the stability of 7 polyphenols during in vitro digestion. The changes
in seven polyphenol standards at different phases of gastrointestinal (GI) digestion were estimated by an HPLC method.
The results showed that no significant changes were observed in phenolic acid compounds in gastric digestion; however,
flavonoids compounds decreased significantly. Except ferulic acid and р-hydroxy benzoic acid, the amounts of all phenolic
compounds investigated were decreased significantly in intestinal phase and at the same time, chlorogenic acid were
transformed into new compounds. The mixed standards had no significant changes during gastric digestion. However,
epicatechin and gallic acid were significantly reduced to 76.96% and 50.30%, respectively after intestinal digestion, while
5 other phenolic compounds did not change noticeably.

Interaction Properties of Caffeoylquinic Acid Derivatives from Ilex kudingcha C. J. Tseng with α-Amylase
XU Donglan, WANG Qingchuan, ZENG Xiaoxiong, SUN Yi
2016, 37(13):  6-12.  doi:10.7506/spkx1002-6630-201613002
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The inhibitory effects of six caffeoylquinic acid (CQA) derivatives (3-CQA, 4-CQA, 5-CQA, 3,4-diCQA,
3,5-diCQA and 4,5-diCQA) against α-amylase were studied comparatively by inhibitory activity assay. Furthermore, the
potential interaction mechanisms between CQA derivative and α-amylase were investigated by fluorescence quenching and
circular dichroism (CD) spectroscopy. The binding parameters were calculated according to modified Stern-Volmer equation,
and the thermodynamic parameters were determined by the van’t Hoff equation. The results showed that all CQA derivatives
exhibited inhibitory effects on α-amylase and the half inhibitory concentrations (IC50) were 1.54, 1.05, 1.28, 0.96, 0.33 and
0.64 mg/mL, respectively. CQA derivatives interacted with α-amylase, forming stable complexes and leading to fluorescence
quenching. Thermodynamic analysis indicated that the interaction process was spontaneous, and hydrophobic force might
be primarily responsible for the interaction. In addition, the CD spectra suggested that the binding of CQA derivatives to the
enzyme induced the change of protein structure, thus destabilizing the enzyme and reducing its activity.

Antioxidant Activities of Anthocyanins from ‘Jianchuanhong’ and ‘Zhuanxinwu’ Pigmented Potatoes
XIAO Jiping, YANG Xiaoyan, GUO Huachun
2016, 37(13):  13-18.  doi:10.7506/spkx1002-6630-201613003
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This study comparatively evaluated the antioxidant activities of individual and combined anthocyanins extracted from the tubers of the red-skinned and red-fleshed potato variety ‘Jianchuanhong’ and the blue-skinned and blue-fleshed potato variety ‘Zhuanxinwu’, both of which are rich in anthocyanins as important natural antioxidants, with the aim of achieving better exploitation and utilization of these low-cost, easily available agricultural materials. The antioxidant activities were tested using four different in vitro models such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2’-azinobis-(3-ethylbenzthiazoline-6-sμlphonate) radical (ABTS+·), hydroxyl (·OH) and superoxide anion free radical (O2-·)scavenging assays with vitamin C (VC) as a control. The results showed that anthocyanins extracted from ‘Jianchuanhong’ and ‘Zhuanxinwu’ potatoes had strong antioxidant activity in a concentration dependent manner and revealed a significant
free radical scavenging capacity 10.7–31.3 times higher than that of VC. For both pigmented potato varieties, the capacity for scavenging four kinds of free radical revealed the following order: ABTS· > DPPH radical > O2-·> ·OH. The antioxidant activity of anthocyanins from ‘Zhuanxinwu’ potato was better than that of ‘Jianchuanhong’ potato. Anthocyanins from both varieties, in combination, were not antagonistic but were synergistic in scavenging free radicals, and the synergistic effects against different free radicals were different.

Antioxidant Activity and Preliminary Structure Analysis of Polysaccharides from Cordyceps cicadas
FENG Yan, GONG Xiaohui, WEI Dequn, LIU Yingkun, ZHAO Ming, YU Xiaofeng, OPEYEMI Joshua Olatunji,JIAO Xinyi, OUYANG Zhen
2016, 37(13):  19-24.  doi:10.7506/spkx1002-6630-201613004
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In this study, the antioxidant activities and structural characteristics of polysaccharides from Cordyceps cicadae
were determined. Two crude polysaccharide fractions (CP50 and CP80) were extracted from Cordyceps cicadae with hot
water and isolated by precipitation with different concentrations of ethanol. Their antioxidant activities were also analyzed.
The results indicated that both CP50 and CP80 were effective in antioxidant activity. The radical scavenging activity of
CP50 was higher than that of CP80. CP50 also showed higher reducing capacity and total antioxidant activity. CP50 was then
further purified by gel filtration on diethylaminoethyl (DEAE)-cellulose-52 and Sephadex G-100 columns, and two purified
fractions were obtained, namely CPA-1 and CPB-1. Ultraviolet spectroscopy and high-performance liquid chromatography
(HPLC) analysis revealed that CPA-1 and CPB-1 were homogeneous. Monosaccharide analysis revealed that both CPA-1
and CPB-1 were composed of Glc, Gal and Man with a molar ratio of 1:0.48:0.52 and 1:0.14:0.114, respectively. Moreover,
both polysaccharide fractions had three helix structures.

Separation of Melanoidin from Shanxi Aged Vinegar and Its Antibacterial Activity
GUO Liyun, YANG Xiaolan
2016, 37(13):  25-30.  doi:10.7506/spkx1002-6630-201613005
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Shanxi aged vinegar extract (SAVE, lyophilized aqueous extract) was separated by ultrafiltration into different
molecular weight aqueous soluble fractions and the high molecular weight fraction was further purified into melanoidins.
Thereafter, the melanoidins were dissociated in aqueous NaCl solution into two components: skeleton and small molecular
weight complex. The antibacterial activity of these components against E. coli, Staphylococcus aureus and Bacillus subtilis
was determined by a turbidimetric method. Results indicated that melanoidins from SAVE had a significant antibacterial
activity (P < 0.05), and they played a major role in the bacteriostatic activity of SAVE. The antimicrobial activity of the
melanoidin component with molecular weight of 3?5 kD was the strongest, with minimal inhibitory concentration (MIC)
of 5 mg/mL for all three bacteria tested. The small molecular weight complex from melanoidin had greater bacteriostastic
activity than the skeleton part.

Effects of Amino Acids and Reducing Sugars on Maillard Reaction Products
QIAN Min, BAI Weidong, ZHAO Wenhong, LIU Xiaoyan, ZENG Xiaofang
2016, 37(13):  31-35.  doi:10.7506/spkx1002-6630-201613006
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The aim of this study was to monitor aroma and hazardous compounds in Maillard reaction products (MRPs)
derived from model systems consisting of amino acids (cysteine, glycine and glutamic) and reducing sugars (xylose and
glucose). The results showed that more kinds of amino acids participating in the Maillard reaction could result in the
formation of more volatile compounds and more abundant flavors. The reaction in the presence of glycine could generate
less acrylamide, while a higher amount of acrylamide could be formed in the presence of glutamic acid. The type of reducing
sugar had no impact on the generation of acrylamide. Higher content of 5-hydroxy methylfurfural (5-HMF) in MRPs was
derived from glycine model system, while model systems containing cysteine, glycine or xylose produced lower content of
5-HMF. The amount of acrylamide and 5-HMF was in the safe range under our experimental conditions.

Antioxidant Activities in Vitro and in Cells and Amino Acid Composition of Rapeseed Peptides
ZHANG Jing, ZHANG Yiyi, XU Feiran, SHI Jiayi, JU Xingrong, WANG Lifeng
2016, 37(13):  36-41.  doi:10.7506/spkx1002-6630-201613007
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The rapeseed protein hydrolysate (RPH) obtained by enzymatic hydrolysis of double-zero rapeseed with Alcalase
2.4L was separated by ultrafiltration and gel filtration chromatography to produce active peptide fractions. The antioxidant
activity of RPH and its active peptides was evaluated in vitro. Furthermore, the amino acid composition of the most potent
antioxidant fraction was analyzed. The results showed that RPH was separated into 4 fractions in different molecular weight
ranges. RPH-P4 (molecular weight < 3 kD) had the strongest antioxidant capacity among these peptide fractions. RPH-P4 was
further separated by gel filtration on Sephadex G-15 into 3 peaks. The antioxidant capacity of RPH-P4-S3 was far higher than
that of 2 other peaks (P < 0.05). Its oxygen radical absorbance capacity (ORAC) value was (1 951.90 ± 20.35) μmol TE/g
sample, cellular antioxidant activity (CAA) value (143.38 ± 4.11) μmol QE/g sample, and EC50 value (45.63 ± 3.67) μg/mL.
The content of antioxidant amino acids in RPH-P4-S3 was as high as 72.90%, and the essential amino acid content was
53.52%. This research suggests that RPH-P4-S3, produced by enzymatic hydrolysis of double-zero rapeseed with Alcalase
2.4L, has high antioxidant activity and nutritional value and thus can be applied in the development of antioxidant-related
functional foods and healthcare products as a functional ingredient.

Effect of Tea Polyphenols on Rheological Properties of High-Gluten Wheat Dough
ZHANG Haihua, ZHU Yuejin, ZHANG Shikang, XU Zhong, SHI Peng, HUANG Yunyun, LI Dawei
2016, 37(13):  42-46.  doi:10.7506/spkx1002-6630-201613008
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The rheological properties of high-gluten wheat dough with addition of tea polyphenols (TPs) were explored
with farinograph, extensograph, glutograph and AR-G2 rheometer. Wheat dough rheological properties showed TPs content
dependence. The addition of 0.5% TPs resulted in an increase in dough formation time, stability time, stretch resistance,
stretch ratio, shear relaxation angle and viscoelastic moduli. Nevertheless, dough formation time, relaxation angle and
viscoelastic moduli declined upon the addition of 1.0% TPs. By further increasing the amount of TPs added to 2.0% and 3.0%,
almost all indexes were reduced to levels lower than those observed with the addition of 0.5% TPs. This phenomenon may
be hypothetically due to the reducibility of TPs and the formation of TP-gluten protein complex.

Preparation and Characterization of Ethanol Extract of Propolis Liposomes
GUO Xiali, LAN Yahui, ZOU Yihong, LI Xiong, LUO Liping
2016, 37(13):  47-52.  doi:10.7506/spkx1002-6630-201613009
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The thin film evaporation-dynamic high pressure microfluidization method was used to prepare ethanol extract
of propolis (EEP) liposomes with soybean lecithin and cholesterol as membrane materials. The characterization of EEP
liposomes was examined by dynamic light scattering particle size analyzer, transmission electron microscopy, DPPH radical
scavenging activity assay, and low temperature storage test. The results showed that the average size of the liposomes
obtained, evenly spherical in appearance, was (78 ± 14) nm, the encapsulation efficiency (97.80 ± 5.21)%, and the stability
parameter Ke value (2.53 ± 0.05)%. After the liposomes were incubated at 4 ℃ for 90 and 180 days, their encapsulation
efficiency was reduced by 16.74% and 28.77%, along with a simultaneous increase in polydispersity index (PDI) of 6.61%
and 14.32%, respectively. Moreover, antioxidant activity index (AAI) values for DPPH radical scavenging activity of the
stored liposomes were 2.78 and 2.71, respectively. The contents of 7 compounds in the liposomes changed by 1.08%–
6.63% after 180 days storage as revealed by HPLC analysis compared to 5.55%–19.42% in EEP. This study highlights the
protection of liposome on EEP.

Effect of Exercise on Broiler Meat Quality and Muscle Fiber Development
CAI Jieqiong, ZHENG Yue, ZHU Haopeng, CHEN Zhiqiang, YAN Peishi
2016, 37(13):  53-59.  doi:10.7506/spkx1002-6630-201613010
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This study aims to analyze the impact of exercise on meat quality and muscle fiber development in broilers.
Totally 36 3-week-old Arbor Acres (Arbor Acres, AA) broilers were selected and divided into two groups according to
similar body weight: control and exercise groups. The experiment lasted 6 weeks (from 4 to 9 weeks of age). The amount
of daytime exercise, muscle quality and muscle fiber characteristics were measured at 5, 7 and 9 weeks of age. The results
showed that the amount of daytime exercise per week of the continuous exercise group was significantly higher than that of
the control group (P < 0.05). The broilers in the continuous exercise group followed a clear rhythm, having a rest at noon.
At the age of 9 weeks, breast muscle L* and a* values, leg muscle a* value and muscle fiber diameter of the exercise group
were significantly higher those of the control group (P < 0.05); meanwhile, leg muscle fiber density of the exercise group
was significantly lower than that of the control group (P < 0.05). Therefore, forced exercise can increase broiler daytime
activity level to improve meat color, and promote the development of broiler leg muscle fiber.

Separation, Purification, and Purity Identification of Cd-Binding Protein from Rice
CHEN Lu, CHEN Jiwang, CAI Jun, DING Wenping, WU Yongning
2016, 37(13):  60-64.  doi:10.7506/spkx1002-6630-201613011
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A graphite furnace atomic absorption spectrometry (GFAAS) method was used to determine the cadmium content
in rice grains from different varieties and at milling levels, as well as in four rice seed storage proteins (albumin, globulin,
prolamin and glutelin) obtained through the Osborne sequential extraction method. Moreover, rice cadmium-binding protein
(RCBP) was separated and purified to homogeneity by using ultrafiltration and ion exchange chromatography. The purity and
molecular mass of RCBP were identified. The results showed that the cadmium content in indica rice was higher than that in
glutinous rice and japonica rice and the cadmium content in rice decreased with the increase of milling level. The cadmium
contents of rice albumin, globulin, prolamin, and glutelin were 0.66, 0.31, 0.63 and 0.23 μg/g, respectively. RCBP (fraction c)
with high purity and good homogeneity as well as molecular mass of 14 kD was prepared from rice albumin (RA).

Kinetics of Non-Enzymatic Browning in Low-Salt Pickled Radish
LIU Wei, ZHANG Mingmei, SONG Ying, YANG Manqian, LAN Yang, GOU Zhenqiong, DONG Quan
2016, 37(13):  65-70.  doi:10.7506/spkx1002-6630-201613012
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The paper discusses the causes and kinetics of non-enzymatic browning during pickling of radish, which is
expected to be helpful for the processing of radish. The radish was fermented spontaneously at different temperatures (25,
30 and 35 ℃) in this study. Changes in qualities related to non-enzymatic browning include total polyphenols, reducing
sugar, vitamin C, free amino acid, 5-hydroxymethylfurfural (5-HMF), browning degree (BD) and color parameters (L*, a*
and b*) were studied and fitted with zero order and first order models, respectively. The browning of pickled radish was
non-enzymatic browning, which was mainly due to Maillard reaction and oxidative polymerization. The first order model
could better explain the kinetic changes of total polyphenols, reducing sugar, and free amino acids in radish during pickling.
The changes of 5-HMF and BD accorded with zero order model, and a good correlation between 5-HMF and BD was
observed (R2 > 0.936 0). Color parameters (L*, and b*) could be expressed by a first-order kinetic equation (R2 > 0.801 2).
The fermentation temperature had a significant effect on the quality of pickled radish products, and the quality of pickled
radish can be improved by controlling fermentation temperature.

Effect of Cold Treatment of Grapes on Aroma Components of Ecolly Dry White Wine
LI Nana, WANG Hua, TANG Guodong, YANG Jihong
2016, 37(13):  71-76.  doi:10.7506/spkx1002-6630-201613013
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Objective: To seek the optimum cold treatment for Ecolly grapes for aroma enhancement in Ecolly dry white
wine. Methods: Ecolly grapes were treated at temperature gradients of 4, −8, −20, and −32 ℃ (16 ℃ was used as control),
respectively before winemaking in small containers according to the grape wine making technical specifications, and physical
and chemical analyses of the finished wines were done. Aroma components of the wines were extracted by stir bar sorptive
extraction (SBSE) for qualitative and quantitative analysis by thermal desorption-gas chromatography-mass spectrometry.
Principal component analysis (PCA) was utilized to analyze the aroma components through SPSS 19.0 software. Eight
trained sensory panelists evaluated the sensory quality of these dry white wine samples. Results: Low temperature treatment
caused an increase in the kind and amounts of aroma components in wine. Conclusion: Low temperature treatments at either
−8 or −20 ℃ can significantly improve the quality of wine aroma by enhancing its aroma intensity to some extent.

Antioxidant Activity and Inhibitory Activity of Ethanol Extract from Ficus auriculata Fruits against α-Glucosidase and Acetylcholine Esterase
TAN Lin, ZHENG Xiaoyan, WANG Jiashui, MA Funing, KANG Youfa, ZANG Xiaoping, MA Weihong
2016, 37(13):  77-81.  doi:10.7506/spkx1002-6630-201613014
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Total phenolics were extracted from Ficus auriculata fruits using 65% ethanol and quantified by the Folin phenol
method. The antioxidant activity of the ethanol extract was assessed by DPPH, ABTS and hydroxyl radical scavenging
assays and its inhibitory activity against alpha-glucosidase and acetylcholine esterase was also evaluated. Results showed
that the fruit extract contained high levels of total phenolics (1.92 mg/g DW) and could scavenge DPPH, ABTS and hydroxyl
radicals effectively in a concentration dependent manner with IC50 of 141.25, 91.20 and 45.71 μg/mL, respectively. It also
exhibited strong inhibitory activity against α-glucosidase in a concentration dependent manner with IC50 of 102.33 μg/mL.
Similarly, it showed inhibitory activity against acetylcholine esterase with IC50 of 4.9 mg/mL. Therefore, the polyphenolrich
extract from Ficus auriculata fruits has strong antioxidant potential and inhibitory activity against α-glucosidase and
acetylcholine esterase.

Application of Se-Fertilizer Affects Selenium Content, Cadmium and Lead Accumulation and Fruit Quality in Kiwifruits
LONG Youhua, ZHANG Cheng, WU Xiaomao, LI Ming, YAO Xue, SHAO Shuang
2016, 37(13):  82-88.  doi:10.7506/spkx1002-6630-201613015
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The aim of the present study was to evaluate the effect of exogenous selenium (Se) on Se content, cadmium (Cd)
and lead (Pb) accumulation, and internal quality of the kiwifruit cultivar ‘Guichang’. The kiwifruit plants growing under
conventional conditions were supplied with sodium selenite at 0.00, 1.25, 2.50, 5.00, 10.00 or 20.00 mg/kg in irrigation water
and organic fertilizer, respectively, via soil deep-ditch fertilization. The results showed that the application of exogenous
Se in both irrigation water and organic fertilizer could increase Se content, decrease Cd and Pb accumulation, and improve
fruit quality in kiwifruits. Moreover, the supplement of sodium selenite was more effective in organic fertilizer than in irrigation
water. With increasing amount of Se supplement, Se content in kiwifruits gradually increased and Cd and Pb contents in kiwifruits
sharply decreased. At sodium selenite dose levels of 1.25–10.00 mg/kg, the contents of vitamin C, soluble sugar, soluble solid
and dry matter were increased, and titratable acid was decreased in kiwifruit. Sodium selenite in organic fertilizer at dose levels of
5.00–10.00 mg/kg was recommended for cultivation of the kiwifruit cultivar ‘Guichang’. These findings might have practical
implications for the development of selenium-enriched kiwifruits and remediation of soil heavy metal contamination.

Isolation, Purification and Antioxidant Activity of Polysaccharides from Zizyphus jujube cv. Dongzao
PAN Ying, XU Jingwei
2016, 37(13):  89-94.  doi:10.7506/spkx1002-6630-201613016
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Objective: To study the monosaccharide composition, structure and antioxidant activity of polysaccharide
fractions isolated and purified from Zizyphus jujube cv. Dongzao. Methods: The extraction of polysaccharides was performed
by water extraction and subsequent ethanol precipitation, and after removal of proteins and pigments, the polysaccharides
were purified by successive DEAE-52 cellulose column and Sephadex G-100 gel column chromatography. The purity
and molecular weights of the purified polysaccharides were determined by Sephadex G-100 column chromatography.
Preliminary structural characterization was carried out by ultraviolet-visible (UV-VIS) spectroscopy, infrared (IR)
spectroscopy and gas chromatography (GC). The antioxidant activity of the crude and purified polysaccharides was evaluated
using the phenanthroline method and 1, 1-diphenyl-2-picrylhydroxyl (DPPH) radical scavenging activity assay. Results:
Two polysaccharide fractions (DPA and DPB) were purified to homogeneity from the crude polysaccharides as identified by
Sephadex G-100. The molecular weight of DPA and DPB was 1.04 × 104 and 3.02 × 105 D, respectively. DPA and DPB did
not contain protein or nucleic acid, but contained the pyranose ring skeleton glucoside. The monosaccharide composition
of DPA consisted of arabinose, mannose, glucose and galactose with a molar ratio of 6.66:1.00:6.75:2.09, whereas that of
DPB consisted of rhamnose, arabinose, mannose, glucose and galactose with a molar ratio of 4.33:10.90:1.00:3.25:4.78.
The crude polysaccharides, DPA and DPB all had antioxidant activity in a concentration dependent manner. The scavenging
percentages of DPA and DPB at 8 mg/mL for hydroxyl radical were 28.52% and 78.79% respectively, which were 9.97%
and 24.54% at 0.4 mg/mL, respectively. Conclusion: Both DPA and DPB had antioxidant activity.

Effect of Different Processing Conditions on the Quality of Hot Pot Soup Base
WANG Qiang, WANG Rui, XIONG Zhengwei, SONG Zhenwei, REN Yanrong
2016, 37(13):  95-100.  doi:10.7506/spkx1002-6630-201613017
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The effect of different processing conditions (temperature, pressure and heating area) on the color, soluble
solids, moisture content and sensory quality of hot pot soup base was studied in this investigation. The results showed
that color parameters b* and L* increased by 24.2% and 19.9%, respectively (P < 0.05) with the increase in pressure
(0.1?0.5 MPa), while the optical rotation value at 0.5 MPa decreased by 13.2% compared with that at atmospheric pressure
(0.1 MPa). At pressures higher than 100 MPa, L* and b* values were significantly lower than those at 0.1?0.5 MPa, indicating
that the low pressure was favorable for the chroma and dry matter leaching of samples. Compared with normal pressure,
0.25 MPa pressure effectively improved the product taste, and obtained higher sensory score. On the other hand, heating area
and temperature had no significant effect on hot pot soup base color or soup concentration (P > 0.05). These findings are
of important significance for improving the utilization rate of hot pot seasonings and reducing the cost of raw materials in
practical production.

Effect of Addition of Different Amounts of Raw Materials with Low Glycemic Index and Wheat Gluten on Wheat Dough Rheological Properties
CHEN Bing, TIAN Baoming, ZHANG Jing, LIU Xiong
2016, 37(13):  101-106.  doi:10.7506/spkx1002-6630-201613018
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The one-factor-at-a-time method was adopted to investigate the effect of adding different amounts of low glycemic
index (GI) raw materials (konjac refined powder, microcrystalline cellulose and high amylase corn starch) and wheat gluten
to wheat flour on the farinograph and extensograph characteristics of dough. The results showed that the addition of konjac
refined powder was found to be helpful to improve the quality and farinograph and extensograph characteristics of the
blended flour. By contrast, microcrystalline cellulose decreased farinograph and extensograph properties, having a negative
impact on dough quality. Furthermore, its addition amount should be controlled below 9%. The addition of high amylase
corn starch weakened farinograph properties, but it when added at 6%?9% improved extensograph characteristics. Wheat
gluten at proper levels 4%?6% could simultaneously improve both farinograph and the extensograph characteristics.

Cultivar Selection for Isoflavone Accumulation and Kinetics of Soybean Germination under UV-B Radiation
CHEN Xu, YANG Runqiang, GU Zhenxin
2016, 37(13):  107-113.  doi:10.7506/spkx1002-6630-201613019
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Soybean cultivars Tian Jiao (TJ), Su Qing1 (SQ1), Su Qing3 (SQ3) and 95You1 (95Y1) were germinated
for 5 days under UV-B radiation to select the best cultivar for isoflavone accumulation based on the content of
isoflavone and the activity of key enzymes and comprehensive analysis of physio-biochemical indexes. Results
showed that sprouts of the TJ cultivar had the highest levels of isoflavone content, phenylalnine ammonialyase
(PAL) activity, and chalcone synthase (CHS) activity as well as the best quality. Meanwhile, the germination
percentage, sprout length, respiratory rate, and the contents of free amino acids and reduced sugar of the cultivar
TJ were higher than those of other tested cultivars. Thus, the cultivar TJ could be considered as a good source
for isoflavone accumulation. The content of isoflavone significantly increased with germination time, which was
3.02 times higher at 7 d than at 0 d. The activity of PAL likewise increased while the activity of CHS first increased and then
decreased. Macromolecular compounds such as soluble protein trended to decrease whereas small molecules such as free
amino acids increased gradually. In conclusion, TJ was screened out for isoflavone accumulation under UV-B radiation.

Bioengineering
Screening of Monacolin K-Producing Monascus and Effects of Chinese Medicinal Herbal Materials on Monacolin K Production in Solid-State Fermentation
CHE Xin, MAO Jian, LIU Shuangping, ZHOU Zhilei, XUE Jingbo
2016, 37(13):  114-119.  doi:10.7506/spkx1002-6630-201613020
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One strain with the most powerful capability of producing Monacolin K named M2-1 was screened from red
mold rice (RMR) using Monacolin K-containing plates and validated by solid-state fermentation. The yield of Monacolin K
reached 1.472 mg/g (dry basis) after 12 d fermentation. The strain was identified as Monascus ruber by morphological and
rDNA-ITS sequence analysis. The effects of powders and water extracts of 15 edible and medicinal herbs on Monacolin K
production and color value of M2-1 in solid-state fermentation were studied. Results indicated that Pericarpium Citri
Reticulatae, Fructus Crataegi, Folium Mori and Radix Angelicae Dahuricae powder as well as Fructus Crataegi, Flos
Caryophylli and Fructus Piperis extracts significantly improved the quality of RMR (P < 0.05). By adding Pericarpium Citri
Reticulatae powder and Fructus Crataegi extract to the fermentation medium, the maximum Monacolin K production of
2.118 mg/g and the highest color value of RMR of 320.12 U/g were obtained which were increased by 43.89% and 71.79%
compared those obtained with the ordinary strain, respectively. This study discovered a variety of Chinese medicinal herbs
that could promote the production of Monacolin K and Monascus pigment by Monascus ruber, which will provide a novel
strategy for improving the efficacy of traditional RMR as an edible and medicinal resource.

Preparation and Characterization of Glucose Oxidase Liposomes
GUAN Huanan, HAN Bolin, NAO Amin, WANG Xin, XU Liping, SUN Lu
2016, 37(13):  120-124.  doi:10.7506/spkx1002-6630-201613021
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Egg phosphatidylcholine and cholesterol were used as coating materials to prepare glucose oxidase (GOD)
liposomes by a thin film evaporation-freezing thawing method. Single factor experiments and orthogonal array design
methods were used to optimize the preparation conditions based on encapsulation efficiency. The shape, stability and
particle size distribution of liposome-encapsulated glucose oxidase were systematically investigated. The optimal process
parameters were obtained as follows: mass ratio between egg phosphatidylcholine and cholesterol of 4:1, 5 mg (500 U) of
GOD, hydration time of 15 min, and 10 freezing-thawing cycles. The encapsulation efficiency of GOD liposomes was up to
(87.7 ± 2.1)%, with an average diameter of approximately (164.75 ± 2.55) μm under the optimized conditions. The encapsulation
efficiency did not significant change at 4 ℃ and room temperature (23.0 ± 0.5) ℃, and the activity of GOD remained stable.

Screening of Exopolysaccharide (EPS)-Producing Lactobacillus plantarum and Bioactivity of the EPS
FENG Xiaowan, XIA Yongjun, WANG Guangqiang, AI Lianzhong
2016, 37(13):  125-129.  doi:10.7506/spkx1002-6630-201613022
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The present study describes the screening of lactic acid bacterial (LAB) strains with high exopolysaccharide
(EPS)-producing ability by observing whether they could form sticky colonies. One strain named AR307 was finally
obtained and identified as Lactobacillus plantarum. In order to enhance EPS yield, the fermentation conditions for producing
EPS by Lactobacillus plantarum AR307 were optimized. The yield of EPS produced by AR307 reached up to 389 mg/L
after 20 h fermentation at 32 ℃. In vitro assay showed that the EPS had good inhibitory activity against colon cancer HT-29
cells and possessed an antidiabetic effect.

Purification and Characterization of Pyrophosphatase from Dorsal Muscle in Silver Carp (Hypophthalmichthys molitrix)
LIU Wei, XU Meng, ZHANG Yawei, ZHOU Li, MA Zhifang, WANG Fulong, PENG Zengqi
2016, 37(13):  130-135.  doi:10.7506/spkx1002-6630-201613023
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Pyrophosphatase (PPase) was extracted and purified from silver carp (Hypophthalmichthys molitrix) dorsal
muscle by crude extraction, 50%–80% saturated ammonium sulfate precipitation and DE-52 anion exchange column
chromatography. The purified enzyme migrated as a single band with a molecular mass of 40 kD on SDS-polyacrylamide
gel electrophoresis (SDS-PAGE). The enzymatic characterization showed that the purified enzyme could hydrolyze
pyrophosphates, and the time range for its initial velocity was 0–15 min. The optimum temperature for the purified PPase
was 45 ℃ and the optimum pH was 7.5. Mg2+ was necessary for PPase activation and the activity reached up to the
maximum value at Mg2+concentration of 5 mmol/L. The activity of PPase was strongly inhibited by 5 mmol/L Ca2+, Zn2+,
EDTA-Na2, EDTA-Na4, KIO3 or 1 mmol/L NaF. The kinetic constants vmax and Km of the purified PPase for tetrasodium
pyrophosphate as substrate were 0.051 U/mg and 0.54 mmol/L, respectively.

PCR-DGGE Analysis of Lactic Acid Bacterial Community Structure in Pickled Mustard Tuber
YAN Pingmei, QIAO Hongping, ZHAO Wenjing, SHAN Shuhua, WANG Qi, CHAI Zheng, CHEN Yanfei
2016, 37(13):  136-139.  doi:10.7506/spkx1002-6630-201613024
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In order to understand the diversity of lactic acid bacteria in pickled mustard tuber and its influencing factors, two
commercial bagged pickled mustard tubers (with and without hot pepper) were determined for the contents of salt and nitrite,
and the V7-V8 region of total bacterial 16S rDNA sequences was separated by denaturing gradient gel electrophoresis
(DGGE). Furthermore, the species richness, evenness and diversity of lactic acid bacteria were analyzed by the Quantity
One software. Results indicated that the contents of salt and nitrite in the first sample were slightly lower than in the second
sample. However, no significant difference in species richness, evenness and diversity was observed between both samples
(P > 0.05). The target DGGE bands were recovered, cloned and sequenced by comparison with the sequences published
in the GenBank after PCR amplification. A total of 5 bands from the DGGE gel of the second sample were recovered and
identified as Pediococcus argentinicus CRL 776, uncultured Lactobacillus sp. isolate DGGE gel band lx12, uncultured
bacterium clone 11.02-12, uncultured bacterium clone 11.02-9, and uncultured Lactobacillus sp. clone PxSC03 with a
similarity of 98%, 96%, 97%, 97%, and 97%, respectively. Four bands from the first sample were recovered and identified
as uncultured Lactobacillus sp., Lactobacillus sakei A156, Lactobacillus sakei YY1, and Lactobacillus sakei WJ1 with a
similarity of 96%, 97%, 98% and 97%, respectively. These results show that hot pepper has little effect on the microbial
community structure of pickled mustard tuber.

Temporal Expression of Staphylococcal Enterotoxin K (sek) Gene in Three Isolates from Food Samples
WANG Qiong, TANG Junni, TANG Cheng, CHEN Juan, LIU Ji, CAI Zijian
2016, 37(13):  140-146.  doi:10.7506/spkx1002-6630-201613025
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Objective: The newly identified staphylococcal enterotoxin sek gene is found to be very popular in clinical
methicillin-resistant S. aureus isolates. The study of sek gene temporal expression would provide some valuable information
and new data for food poisoning prevention and disease control linked to this bacterium. Methods: Three strains (SA005,
SA008 and SAB0) with sek gene from foodborne isolates in our lab were selected by PCR detection of 21 different SEs
genes. The growth curves of three strains were monitored during the growth phase in TSB. Total RNAs from three strains
were extracted at different growth periods, and a quantitative real time-PCR was developed to monitor the relative expression
of sek gene. Results: The results of virulence gene detection showed that SA005 harbored 16S, nuc, mecA, seb, sec, sek and
sex genes, SA008 harbored 16S, nuc, mecA, sea, sek and sex genes, and SAB0 harbored 16S, nuc, sek and sex genes. The
characteristics of growth curves for three strains had similarity, which exhibited a turning point around 18 h, and then entered
the stationary growth phase. mRNA expression levels were shown during all the growth phases (12–120 h); however, there
were very obvious differences among three strains and among different sampling points. The relative expression of sek gene
was very high at 48 h and very low at 84 h for SA005. For SA008, it was very high at 24 h, very low at 48 h, and was stable
between 60–120 h. The relative expression of sek gene in SAB0 presented a parabolic trend, with a high level at 60 h and
72 h (P > 0.05). Conclusion: The relative expression of sek gene among three strains had a significant difference. Even in the
same strain, the relative expression of sek gene was different at different time points. Strain-to-strain variations with different
background genes may be the key points for different expression patterns of sek gene.

Comparison and Evaluation of Models for the Growth of Shewanella putrefaciens at Different Temperatures
ZHU Yanqi, GUO Quanyou, LI Baoguo, JIANG Chaojun
2016, 37(13):  147-152.  doi:10.7506/spkx1002-6630-201613026
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The specific spoilage bacterium Shewanella putrefaciens isolated from refrigerated large yellow croaker was
grown at 5, 8, 15 and 25 ℃ in tryptone soy broth and the modified Gompertz equation, the modified Logistic equation and
the Baranyi equation were applied to fit its growth kinetic models. Further, a second-order model was established using
the Belehradek equation indicating the influence of temperature on the growth kinetics of Shewanella putrefaciens, and the
goodness of fit and applicability of the models were examined. The results showed that temperature remarkably affected the
growth kinetics of Shewanella putrefaciens. The growth of Shewanella putrefaciens was significantly inhibited at 5 ℃ with
a longer lag phase. At temperature up to 25 ℃, the lag phase of growth was reduced significantly. The specific growth rate
increased with temperature. Moreover, linear relationships between temperature and either the specific growth rate or the lag
period of growth were observed. Root mean square error (RMSE), residual sum of squares (RSS), bias factor (BF), accuracy
factor (AF) and R2 were used to evaluate the goodness of fit of the developed models. The results showed that the evaluation
parameters RSS, RMSE of the modified Logistic model were minimal, and BF and AF values were close to 1, suggesting that
the goodness of fit and applicability of the modified Logistic model were the best. Therefore, the modified Logistic model
can be used to predict the growth of Shewanella putrefaciens in aquatic products.

Cloning and Expression of Mannitol Dehydrogenase Gene from Leuconostoc pseudomesenteroides
CHENG Yayun, WANG Xin, ZHENG Lin, LI Guanhao, CUI Hushan, JIN Qing
2016, 37(13):  153-156.  doi:10.7506/spkx1002-6630-201613027
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The structural gene encoding mannitol dehydrogenase (MDH) from Leuconostoc pseudomesenteroides was
amplified by PCR and cloned into the vector pETDuet-1. As a result, the plasmid pETDuet-1-mdh was constructed and
transformed into E. coli BL21(DE3) for MDH expression induced by isopropyl-β-D-thiogalactoside (IPTG). The length
of the mdh structural gene was l 017 bp. The recombinant mdh gene was successfully expressed in Escherichia coli and
the molecular weight of the expressed protein was 36.0 kD. The activity of recombinant mannitol dehydrogenase was
0.15 U/mg pro, which was higher than the MDH activity of Leuconostoc pseudomesenteroides (0.03 U/mg pro).

Isolation and Identification of Lactobacillus salivarius from Chicken Intestine and Its Biological Characteristics
CHEN Xinliang, SHAO Qibing, WANG Chao, HE Jianbin, ZHANG Yi, YANG Shuhua, LI Peng, LONG Miao
2016, 37(13):  157-161.  doi:10.7506/spkx1002-6630-201613028
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An isolate of the anaerobic bacterium Lactobacillus salivarius was obtained from the intestine of chicken and
identified by colony morphology, Gram staining, physio-biochemical reactions, and 16S rRNA sequencing analysis. Results
showed that the strain was Lactobacillus salivarius and named as Lactobacillus salivarius C-1-3. Then the strain was
subjected to antibacterial, cellulose degradation, and acid, bile salt and high temperature tolerance tests. It turned out that
the strain C-1-3 had a powerful antibacterial effect against E. coli, Salmonella and Staphylococcus aureus. And it also had
cellulose degradation ability. After several generations of domestication, this strain possessed good antibacterial performance
and significant tolerance to acid and high temperature.

Enrichment and Isolation of Salmonella enteritidis by Immune Magnetic Nanoparticles
HU Yuxin, ZHENG Shu, HE Zao, LUO Fang, LIU Xia
2016, 37(13):  162-167.  doi:10.7506/spkx1002-6630-201613029
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In this study, carboxyl-coated magnetic namoparticles (MNPs) were prepared using a chemical coprecipitation
method. The coupling conditions for conjugates (immune MNPs) of MNPs and anti-Salmonella polyclonal antibody (PAb)
were optimized, and then the application of immune MNPs in the enrichment and isolation of Salmonella enteritidis was
evaluated, which will provide a more fast and effective way for the detection of Salmonella. The results showed that the
optimal coupling of MNPs and PAb was achieved when the volume ratio of 51.7 μg/mL MNPs to 0.4 mol/L EDC-0.1 mol/L
NHS to 1.0 mg/mL PAb was 1:2:2 in a water bath at 37 ℃ for 40 min. The adsorption efficiency attained 94.36% when the volume
ratio between 51.7 μg/mL immune MNPs and 104 CFU/mL S. enteritidis was 4:5, and the incubation time was 40 min.

Purification and Characterization of Trypsin from White Croaker (Argyrosomus argentatus)
LI Huan, LI Ting, ZHAN Yunchao, DU Cuihong
2016, 37(13):  168-172.  doi:10.7506/spkx1002-6630-201613030
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An anionic trypsin (named as trypsin A) from the pyloric caeca of white croaker (Argyrosomus argentatus) was
purified by ammonium precipitation, DEAE-Sepharose Fast Flow anion exchange chromatography and Sephacryl S-200 gel
filtration chromatography. The characteristics of trypsin A were investigated. The results showed that trypsin A migrated as
a single protein band with a molecular weight of about 28 kD in SDS-PAGE. Trypsin A exhibited its optimal temperature at
50 ℃ and was stable below 65 ℃. Trypsin A revealed its optimal pH at 9.5 and was stable in the pH range from 9.5 to 11.0.
The purified trypsin A was analyzed by western blotting using anti-common carp trypsin antibody, and the result showed
that the active region of the trypsin was highly conservative. Serine proteinase inhibitors were effective against trypsin A.

Preparation of Polypeptides with Sobering Effect from Peanut Meal by Enzymatic Hydrolysis
NING Qingpeng, WANG Changqing, FANG Tian, BAI Yunyun, CHEN Tong
2016, 37(13):  173-177.  doi:10.7506/spkx1002-6630-201613031
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For more reasonable and effective utilization of peanut meal resources, we investigated the preparation of
polypeptides with sobering effect by enzymatic hydrolysis of peanut meal with Alcalase AF 2.4L. Polypeptide fractions were
separated and their sobering effect was evaluated in vitro and in animals. The results showed that the optimal hydrolysis
conditions for preparing polypeptides with sobering effect were determined to be 3 h hydrolysis at 35 ℃ and pH 9.5 with a
solid-to-solvent ratio of 1:30 (m/V). Under these conditions, the percentage activation of alcohol dehydrogenase (ADH) and
peptide yield were significantly improved. The fraction with molecular weight in the range of 1 000?3 000 D had the highest
activating effect on ADH, with a percentage activation of 30.47%. G25 gel permeation chromatographic analysis showed
that the yield of peanut meal peptides with molecular weight less than 3 000 D was 89.55%, giving a percentage activation
of ADH of 29.25%. Animal experiments proved that the peanut meal polypeptides could significantly prevent alcoholism
and sober up drunken mice. They at high dosages could significantly reduce blood alcohol content in mice in 60?90 min.

Nutrition & Hygiene
Effect of Hydrolyzed Wheat Protein Peptide on Ethanol-Induced Acute Gastric Mucosal Damage in Rats
YANG Xian, WANG Yanyan, WANG Feng, XIA Hui, PAN Xingchang, ZHU Hangju, GU Ruizeng, MA Yongqing, TANG Huali, WANG Shaokang, SUN Guiju
2016, 37(13):  178-182.  doi:10.7506/spkx1002-6630-201613032
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Objective: The present work was aimed to examine the protective effect of hydrolyzed wheat protein peptide
(HWP) in the model rats with acute gastric mucosal damage induced by ethanol. Methods: Hydrolyzed wheat protein
peptide (167, 333 or 667 mg/(kg·d) (in body mass), cimetidine (65 mg/(kg·d)), or distilled water were administered daily by
gavage for 30 days before the rats were treated with anhydrous ethanol (1 mL) and then euthanized after 1 h. Histological
observation was performed to evaluate the antioxidant and anti-inflammatory activity of HWP as well as its impact on serum
physiological and biochemical parameters and the expression of caspase-3 and epidermal growth factor receptor (EGFR).
Results: Histological observation showed that ethanol-induced gastric mucosal damage was attenuated by hydrolyzed
wheat protein peptide pretreatment. Hydrolyzed wheat protein peptide remarkably increased the superoxide dismutase
(SOD) activity, epidermal growth factor (EGF) and prostaglandin E-2 (PGE2) levels in serum. Furthermore, it dramatically
decreased malondialdehyde (MDA), platelet-activating factor (PAF) and interleukin-8 (IL-8) levels in serum. The expression
level of caspase-3 in stomach was significantly decreased in hydrolyzed wheat protein peptide-treated rats when compared
with the model group; however, the expression level of EGFR was markedly increased. Conclusion: Ethanol-induced gastric
mucosal damage in rats can be protected against by hydrolyzed wheat protein peptide. The potential mechanism may be
associated with the enhancement of antioxidant, anti-inflammatory and anti-apoptotic functions.

Relationship between Nitrate Contents of Chinese Cabbage (Brassica chinensis L.) and Light Intensity or Key Enzymes of Nitrogen Metabolism
CHEN Yongqin, FENG Bo, XU Weihong, YANG Yun, CHI Sunlin, CHEN Xugen, WANG Zhengyin, XIE Deti
2016, 37(13):  183-188.  doi:10.7506/spkx1002-6630-201613033
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Pot experiment was carried to explore the relationships of nitrate contents with photosynthetic system parameters
in two Chinese cabbage varieties (Brassica chinensis L.) (the low-nitrate variety ‘JN4’ and the high-nitrate variety ‘HKTX’)
under different light intensities (3 000, 6 000 and 12 000 lx) as well as with the key enzymes of nitrogen metabolism in
soil and plants. The results showed that the biomass of Chinese cabbage had an increasing trend with increasing light
intensity. The dry weight of shoots of ‘HKTX’ and ‘JN4’ increased by 10.51%?28.09% under light intensities of 6 000 and
12 000 lx as compared with that under 3 000 lx. The nitrate contents in leaves of the two Chinese cabbage varieties decreased
significantly with increasing light intensity. The nitrate contents in leaf blades and petioles of ‘HKTX’ and ‘JN4’ decreased
respectively by 22.27%?43.32% and 8.21%?31.40% under 6 000 and 12 000 lx as compared with those under 3 000 lx.
There were significantly negative correlations between nitrate content and either net photosynthetic rate (Pn) with correlation
coefficients of −0.686 and −0.833 (P < 0.01) or nitrate reductase (NR) activity in leave blades with correlation coefficients
of −0.719 and −0.696 (P < 0.05) for the varieties ‘HKTX’ and ‘JN4’, respectively. Significantly positive correlations existed
between the content of nitrate in either leaf blades or petioles and NO3--N level in soil with correlation coefficients of 0.692,
0.726, 0.676 and 0.796 for ‘JN4’ and ‘HKTX’ (P < 0.05), respectively. Moreover, significantly negative correlations were
observed between soil NO3--N content and nitrate reductase activity with correlation coefficients of −0.826 and −0.832
(P < 0.01), respectively.

Geometric Isomer Composition of Lycopene from Rat Liver
HUI Bodi, FENG Runyue, GONG Ping
2016, 37(13):  189-193.  doi:10.7506/spkx1002-6630-201613034
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Total lycopene amount and lycopene geometric isomer composition of rat liver were determined in this study,
aiming to lay a foundation for in vivo studies on lycopene metabolism and biological function. In the experiment, male
rats were administrated with tomato extract at a single dose. After 8 h, liver tissues were collected for the extraction of
lycopene. The total amount and geometric isomer composition of lycopene were determined by C30-high performance liquid
chromatography with photodiode array detection (C30-HPLC-PDA). Results indicated that total amount of lycopene from
liver tissues was 92.95 μg/g fresh weight. In comparison with tomato extract, the composition and amount of lycopene
Z-isomers (including 13Z-, 11Z-, 9Z- and 5Z-isomer) were increased in rat liver tissues. This phenomenon is important for
investigating the storage, targeting transfer and biological function of lycopene in liver.

Effect of Citric Acid-Modified Sweet Potato Starch on Meat Quality of Schizothorax prenanti Tchang
XIANG Shiqiong, WU Yinglong
2016, 37(13):  194-198.  doi:10.7506/spkx1002-6630-201613035
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Objective: To explore the effect of citric acid-modified sweet potato starch (CSPS) on meat quality of
Schizothorax prenanti Tchang. Methods: The Schizothorax prenanti Tchang with body weights of approximately 75 g
were divided randomly into five groups with 48 fish in each group. After feeding for 60 days, 8 fish were randomly picked
from each tank and meat quality indicators of Schizothorax prenanti Tchang were determined. Results: The intramuscular
fat content of Schizothorax prenanti Tchang was significantly reduced by dietary addition of CSPS at 14% and 28%
(P < 0.05). The intramuscular pH was significantly increased by dietary supplementation of CSPS at 3.5% but was
significantly reduced at 14% (P < 0.05); however, there were no significant changes in the contents of protein, moisture,
hydroxyproline and collagen or water loss rate. The hardness of muscle was significantly increased by adding CSPS at 7%
and 14% to the bait for Schizothorax prenanti Tchang (P < 0.05), addition of CSPS at 28% to the bait led to a significant
increase in the adhesiveness (P < 0.05), and CSPS at 14% likewise resulted in a significant in the gumminess compared with
the control group (P < 0.05). Moreover, the chewiness of muscle was significantly increased by dietary addition of CSPS at
7% and 14% (P < 0.05). On the other hand, no significant changes were noted in the elasticity, cohesiveness or resilience of
muscle. Conclusion: Dietary supplementation of CSPS at 7% and 14% could result in a reduction in fat content in muscle
of Schizothorax prenanti Tchang and an increase in the hardness, adhesiveness, gumminess, chewiness and compactness of
muscle, therefore improving the meat quality of fish.

Bioengineering
Bioactive Peptide Derived from Oyster Meat from Guangxi Region, China Inhibits Proliferation and Migration of Human Ovarian Carcinoma Cells with Directional High Lymphatic Metastasis
LI Chaozhu, CHEN Yanhui, CHEN Yanhua, LI Danrong
2016, 37(13):  199-203.  doi:10.7506/spkx1002-6630-201613036
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Objective: To study the inhibitory effect of bioactive peptide derived from oyster meat Guangxi region,
China on the proliferation and migration of human ovarian carcinoma cells with directional high lymphatic metastasis
(SKOV3-PM4 cells). Methods: The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay
was performed to study the inhibitory effect of the bioactive peptide on cell proliferation in 24 h. The proliferative ability
of SKOV3-PM4 cells was detected by cell counting method and colony formation test. The ability of cell migration and
invasion was examined by cell wound scratch assay and matrigel invasion assay. Results: MTT results showed that the
bioactive peptide could inhibit the proliferation of SKOV3-PM4 within the concentration range of 5?80 mg/L and that half
inhibition concentration (IC50) in 24 hours was 35.36 mg/L. The cell growth curve and cell colony formation showed that
the proliferative ability of SKOV3-PM4 cells was significantly inhibited (P < 0.05). Compared with control cells, after
being treated with 4 mg/L (non-cytotoxic concentration) the bioactive peptide, the migration and invasion capability of
SKOV3-PM4 was also significantly decreased (P < 0.05). Conclusion: The bioactive peptide derived from oyster from
Guangxi region can inhibit the proliferation and migration of SKOV3-PM4 cells within a certain concentration range.

Nutrition & Hygiene
Bifidobacterium BB12 Promotes Defecation and Regulates Gastrointestinal Hormones in Constipated Rats
TIAN Ying, DAI Qianqian, SHI Minghui, MENG Chanfang, HUANG Yujun, GU Ruixia
2016, 37(13):  204-208.  doi:10.7506/spkx1002-6630-201613037
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Objective: To investigate the effects of Bifidobacterium BB12 on promoting defecation and regulating
gastrointestinal hormones in constipated rats. Methods: Totally 48 Wistar rats, half male and half female, were divided
randomly into control group, positive control group, model group, Bifidobacterium BB12 low-, middle-, and high-dose
groups. Rat models of loperamide hydrochloride-induced constipation were established and the rats from each group were
treated with corresponding drugs. During the experiment, daily food intake, water intake, body weight, fecal number, fecal
water content, gastrointestinal transit, and the contents of gastrin, motilin, substance P and somatostatin in serum were
measured. Results: Fecal number, gastrointestinal transit, and the serum contents of motilin and substance P increased
significantly, while water intake and somatostatin content decreased significantly in response to the administration of
Bifidobacterium BB12. These effects were dependent on gender and dose. There was no significant difference in food intake,
body weight, fecal water content and serum gastrin. Conclusion: The laxative effect of Bifidobacterium BB12 is confirmed
and the underlying mechanism is related to regulating some gastrointestinal hormones.

Antitumor Activity and Mechanism of Action of Wild Cordyceps sobolifera Polysaccharide
XIE Fei, LI Wei, CHEN Meizhen, YU Jie
2016, 37(13):  209-213.  doi:10.7506/spkx1002-6630-201613038
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Objective: The antitumor activity of polysaccharides from wild Cordyceps sobolifera (CSP) was investigated in
vitro and in vivo. Methods: 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay was used to
determine the anti-proliferative effect of CSP at different doses on HeLa cells. S180 sarcoma mouse model was established
and used to detect the antitumor activity and the effect on the immune system of CSP. Results: CSP at concentrations of
400?800 μg/mL inhibited the growth of HeLa cells in vitro in a concentration-dependent manner, with a percentage inhibition
of 61% at 800 μg/mL. In vivo, CSP at both high (200 mg/(kg·d)) and low (100 mg/(kg·d)) doses had antitumor effects, with a
percentage inhibition of 42.9% at 200 mg/(kg·d), and immune organ indices of tumor-burdened mice significantly increased
(P < 0.01). CSP also activated the proliferation and differentiation of lymphocytes, and had an obvious protection on the
antioxidant defense system in the body. Conclusion: CSP has obvious antitumor activity in vitro and in vivo, which may be
related to enhancing the immune system adjustment capacity and antioxidant activity.

Effect of Heat-Moisture Treated Corn Porous Starch on Lipid Metabolism in Rats
LIU Qingqing, YOU Yuming, LU Hongjia, TIAN Baoming, CHEN Zhaojun, LIU Xiong
2016, 37(13):  214-219.  doi:10.7506/spkx1002-6630-201613039
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Objective: To investigate the effect of heat-moisture treated corn porous starch on lipid metabolism in rats.
Methods: Totally 32 female Sprague-Dawley rats were randomly divided into 4 groups including control group and three
other groups as high-fat blank group, high-fat porous starch (PS) group and high-fat heat-moisture treated corn porous starch
(HTMPS) group. After adaptive feeding with a basal diet for a week, the rats from the control group were still fed with the
basal diet and those from high-fat groups were given a high fat diet. After 4 weeks, all rats were sacrificed for the analysis
of triglyceride (TG), cholesterol (TC) in serum and liver, and bile acid in small intestine and feces. Results: By feeding corn
porous starch and heat-moisture treated corn porous starch, the contents of TC, TG and non-HDL-C in serum, atherosclerosis
index (AI) and TG/HDL-C ratio in serum as well as TC content in liver and abdominal fat content decreased significantly
(P < 0.05); the fecal excretion of neutral steroid and bile acid increased significantly (P < 0.05). Compared with HTMPS
group, the rats from PS group revealed a reduction in serum TC level, AI, TG/HDL-C ratio and abdominal fat, and an
increase in fecal bile acid excretion and bile acid in small intestinal contents. Conclusion: PS is more successful in reducing
cholesterol than HTMPS. Therefore, porous starch can reduce the risk of hyperlipidemia caused by high-fat diet, and has a
significant role in preventing cardiovascular diseases.

Reviews
The Hypolipidemic Mechanism of Proanthocyanidins
ZHANG Yu, LI Lanqi, GONG Hui, CHEN Shiguo, YE Xingqian
2016, 37(13):  220-225.  doi:10.7506/spkx1002-6630-201613040
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Proanthocyanidins are a class of polyphenols that consist of flavan-3-ols as their basic unit and exist in various
types of plants. In this review, the regulation of proanthocyanidins on the key proteins of the metabolism pathways of
triglycerides and cholesterol is summarized. Also, how proanthocyanidins regulate body weight, energy intake and energy
expenditure through adenosine 5’-monophosphate-activated protein kinase (AMPK) pathway to exert their hypolipidemic
effects is demonstrated with details as well.

Progress in Research on Biological Activity and Functions of Ribosome-Inactivating Proteins in Bitter Mellon
YANG Guliang, LI Shiming, WANG Shuzhen
2016, 37(13):  226-231.  doi:10.7506/spkx1002-6630-201613041
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Ribosome-inactivating proteins (RIPs) are a family of protein than can remove A4324 from the highly conserved
and surface-exposed sarcin/ricin loop (SRL) in the 28S rRNA of eukaryotic ribosomes. The depurination destroys the
interaction between elongation factors and ribosome, thereby resulting in the inactivation of ribosome and the inhibition of
protein synthesis. RIPs can be classified into type I, type II and type III. The RIPs identified in Momordica charantia L.,
such as α-momorcharin, β-momorcharin, γ-momorcharin, δ-momorcharin, ε-momorcharin and MAP30, all belong to the type
I RIPs. Antiviral, antibacterial, pesticidal and anti-tumor, hypolipidemia, hypoglycemia and other biologically activities have
been reported for these RIPs. In this article, the classification, biologically activity, function and application of RIPs have
been reviewed in the hope of providing a theoretical basis for further development and utilization of bitter mellon RIPs.

Relationship between Small Heat Shock Proteins and Meat Tenderness
LI Xin, XING Tong, XU Xinglian
2016, 37(13):  232-236.  doi:10.7506/spkx1002-6630-201613042
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The development of meat eating quality is closely related to complex biochemical processes during postmortem aging. Oxygen and nutrient withdrawal following exsanguination is known to induce apoptosis, so that muscle cells inevitably engage towards apoptotic cell death. Thus, factors that regulate the process of apoptotic cell death of muscle cells are believed to influence the ultimate meat quality. Small heat shock proteins (sHSPs), as biomarkers for meat quality attributes, have been confirmed by many proteomic studies. Due to the anti-apoptotic and molecular chaperone functions, sHSPs can protect myofibrillar proteins from degradation, interfere with cellular signal transduction pathways, delay myofibrillar protein degradation by endogenous enzymes and extend the process of meat aging. In this review, we discuss the mechanism of their possible chaperone and anti-apoptotic roles involved in meat tenderness during the conversion of muscle to meat

Recent Progress in Research on Antibacterial Action of Oligosaccharides
JI Yaohu, DOU Huating, WU Houjiu, HUANG Linhua
2016, 37(13):  237-242.  doi:10.7506/spkx1002-6630-201613043
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The current situation of food safety in China is still very serious. It is a new task for researchers to seek safe, efficient
and natural food preservative for processed fruits and vegetables. Many studies have proved that oligosaccharides have good
inhibitory effects on the growth of pathogenic microorganisms. The research and application of oligosaccharides as food
preservatives are very significant and promising, which have gradually become a new research hotspot in the food industry. The
present paper reviews recent progress in studying the antibacterial effect and mechanism of oligosaccharides from various sources.
This review is also focused on the current status and future prospects of their application in food preservation.

Progress in Authentication Technologies for Berry Juice
LI Meige, WU Yajun, YANG Yange, XU Xinglian, CHEN Ying
2016, 37(13):  243-250.  doi:10.7506/spkx1002-6630-201613044
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Berry, mainly represented by blueberry and raspberry, has the reputation of golden fruit. Adulteration,
counterfeiting and mislabeling of berry have led to endless disputes on food quality and safety, which poses a great challenge
for identification technologies for berry juice authenticity. Nowadays, conventional physicochemical methods and traditional
molecular biology methods are commonly used to detect the authenticity of berries, all of which have their advantages and
disadvantages. The physicochemical methods are advantageous mainly in quality control and quality evaluation, which,
however, are easy to be influenced by individual growth, processing conditions and other external factors involved in the
recognition of plant-derived ingredients in products. In addition, the molecular biology methods based on DNA are more
commonly used to identify plant-derived ingredients in juice, which complement the physicochemical methods. In this paper,
recent progress in the commonly used technologies for quality control and authentication of berry fruits and juices, mainly
sensory evaluation, physicochemical technologies and molecular biology technologies, is reviewed.

Progress in Hybrid-Hybridoma Technology to Produce Bispecific Monoclonal Antibody and Its Application in Food Safety Detection
WANG Feng, WANG Yu, WANG Hong, XIAO Zhili
2016, 37(13):  251-256.  doi:10.7506/spkx1002-6630-201613045
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Bispecific monoclonal antibodies (BsMAb) are monoclonal antibody molecules with two different specific
antigen-binding sites. Hybrid-hybridoma technology has a great advantage in the preparation of BsMAb because of its easy
operation and less time consumption. BsMAbs with 2 distinct antigen-binding sites produced by the hybrid-hybridoma
technology could specifically recognize antigens of 2 drugs or 2 classes of drugs, and are applicable in the multi-residue
determination of foods. In this paper, we elaborate recent progress in the development of hybrid-hybridoma technology and
explore the mechanism of BsMAb generation by the hybrid-hybridoma method. Several important factors influencing the
hybrid-hybridoma technology are discussed, and the application of BsMAb in food safety detection is also reviewed. This
paper will provide some references for the development and application of BsMAb in multi-residue immunoassay of foods.

Advances in Generation of Reactive Oxygen Species Associated with Health and Disease
LI Xingtai, ZHANG Chunying, ZHONG Weili, GAO Mingbo
2016, 37(13):  257-270.  doi:10.7506/spkx1002-6630-201613046
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Historically, reactive oxygen species (ROS) are thought to exclusively cause cellular damage and physiological
dysfunctions. The accumulation of ROS and oxidative damage has been linked to multiple pathologies, including
neurodegenerative diseases, diabetes, cancer, and premature aging. Accumulating evidence in recent years suggests that
ROS are critical for health. In physiological conditions, ROS are considered as a signaling molecule produced at low level
and in a transient manner. On the other hand, over-production of ROS, associated with the dysfunction of ROS buffering
systems, can lead to the development of chronic diseases. Thus, chronic ROS release participates in the occurrence of over
100 kinds of diseases, including major diseases that threaten human health, such as cancer, cardiovascular diseases, diabetes,
neurodegenerative diseases (Parkinson’s and Alzheimer’s diseases), acquired immune deficiency syndrome, stroke and aging.
This paper reviewes the latest progress in the presence of oxygen on earth and the evolution of aerobic eukaryotic organisms,
ROS types, physiological and pathological pleiotropy of ROS, the major ROS in the body, the generation and reactions of
ROS, the association of ROS with mitochondrial oxidative phosphorylation, apoptosis, immune defense, inflammation and
cancers. Given the importance of ATP and ROS to life, we propose that the balance between the generation of ATP and ROS
by regulation of mitochondrial oxidative phosphorylation will be the key to human health.

Authorized Legislation of Food Safety
HUO Jingyu, LU Lin
2016, 37(13):  271-276.  doi:10.7506/spkx1002-6630-201613047
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The revision of the Food Safety Law authorized local legislation concerning “Three Smalls” (small food
workshops, small vendors and small restaurants). This mode is conducive to the flexibility of local legislation and fluency
of law enforcement. But due to being constrained by the Legislation Law’s strict provisions on authorized legislation, it
does not make a provision about objectives, principles, scope and duration. This kind of regulation always lacks legitimacy.
Therefore, this article suggests making clear the connotation and denotation of the law concept of “Three Smalls”,
establishing the accessibility and punishment mechanisms, enlarging the scope of public participation in local legislation and
promoting the authorized legislation concerning “Three Smalls”.

Current Status of Research on Nanoparticles Containing Fruit Bioactive Compounds
HU Yan, ZHOU Zhiqin
2016, 37(13):  277-286.  doi:10.7506/spkx1002-6630-201613048
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Fresh fruits and their derived products are rich in nutrients and various bioactive compounds which are important
to human health. However, many problems exist with fruit bioactives, such as low solubility, easy degradation, instability,
slow absorption and weak bioavailability. Nanotechnology can overcome these defects effectively. In this paper, the current
status of research on nanoparticles containing fruit bioactive compounds is reviewed. The principles, techniques and methods
for preparing fruit bioactive nanoparticles are discussed. Their biological functions and applications in foods, medicines and
health products and cosmetics are systematically summarized. In addition, existing problems and future research directions
are proposed with an attempt to provide new information on the utilization of fruit bioactive compounds.

Bibliometric Analysis of the Literature Concerning Research on Kiwi Fruits Based on SCI-E
ZHU Changju, PENG Fenglan, LU Mingfang
2016, 37(13):  287-292.  doi:10.7506/spkx1002-6630-201613049
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Totally 1 253 papers reporting studies on kiwi fruit published during 2004–2013 were retrieved from the American Science Citation Index Database (SCI-E) and bibliometrically analyzed with respect to publishing year, literature type, language, subject area, source journals, core author affiliations, national or regional distribution and authorship. The results showed that a growing number of SCI papers focused on studies on kiwi fruit were published over those 10 years. Most of those papers were published in peer-reviewed journals in English, reporting studies in food technology and plant science. Postharvest Biology and Technology published the largest number of articles. The most productive institution was New Zealand Institute for Plant Food Research Ltd.. More papers from New Zealand, Italy and China were discovered in the database. The most productive authors were Gavrovic-Jankulovic, from Serbia, and Clearwater, from New Zealand. Tamburrini, from Italy, was the most influential author. This bibliometric analysis can provide helpful information for scientists in our country to understand present trends in research on kiwi fruit, establish research directions and develop research and academic exchange.